5X Concentrate COOMASSIE^nano Protein Staining Solution

5X Concentrate COOMASSIE^nano -Innovatively developed Next-Generation Protein Stain for faster and environment-friendly gel visualization. 5X COOMASSIE^nano, innovatively enhanced by the proprietary nano-technology, is a concentrated, easy-toreconstitute protein staining solution for SDS-PAGE gels and is also compatible with mass spectrophotometry. Its next generation formula offers faster protein detection and higher sensitivity while eliminating the conventional need for destaining and wash. In the absence of hazardous substances, such as methanol and acetic acid, 5X COOMASSIE^nano is considered to be safe and environmentally friendly by reducing carbon footprint by as much as 80%.

1. Prepare a 5 L bottle.
2. Unpack PS002-L05X and pour the liquid content into the bottle.
3. Rinse residual reagent in the original bottle with deionized water.
4. Add deionized water until the volume reaches 5 L.

1. Make 5X Concentrate COOMASSIE^nano into 1X COOMASSIE^nano protein staining solution.
2. Carefully mix COOMASSIE^nano up and down few times before use.
3. After SDS-PAGE has finished, remove the gel from the cassette and submerge it in the proper amount of COOMASSIE^nano stain, enough to cover the gel. Lightly agitate the staining box for 10 to 30 minutes at room temperature.
4. As there is no need for washing or destaining, please continue to carefully remove the staining solution and rinse the excess stain in the gel with water.
5. Image the gel.

COOMASSIE^nano Protein Staining Solution practices the fast-signal representing at 5 min. Prestained protein ladder, unstain protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS-PAGE (4-20% Novex Gel), please remove the gel from the cassette then proceed to submerge the gel in proper amount of COOMASSIEnano dye, enough to cover the gel. Lightly agitate the staining box for 5 minutes to over night at room temperature.

COOMASSIE^nano Protein Staining Solution demonstrates the high sensitivity detection could be down to 10 ng (Lane H). Prestained protein ladder, unstained protein ladder, and serial diluted BSA were prepared and applied in electrophoresis. After running 10% homemade gel (0.75 mm thickness), please remove the gel from the cassette then proceed to submerge the gel in a proper amount of COOMASSIE^nano Protein Staining Solution, enough to cover the gel. Lightly agitate the staining container at room temperature when staining for 30 hrs.

Important notes

• Container: box for gel staining
• Shaker: orbital or rocking shaker