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Functionally Active Membrane Proteins Incorporated in Mesostructured Silica Films

Functionally Active Membrane Proteins Incorporated in Mesostructured Silica Films

Justin P Jahnke, Matthew N Idso, Sunyia Hussain, Matthias J N Junk, Julia M Fisher, David D Phan, Songi Han, Bradley F Chmelka

J Am Chem Soc. 2018 Mar 21;140(11):3892-3906.

PMID: 29533066

Abstract:

A versatile synthetic protocol is reported that allows high concentrations of functionally active membrane proteins to be incorporated in mesostructured silica materials. Judicious selections of solvent, surfactant, silica precursor species, and synthesis conditions enable membrane proteins to be stabilized in solution and during subsequent coassembly into silica-surfactant composites with nano- and mesoscale order. This was demonstrated by using a combination of nonionic ( n-dodecyl-β-d-maltoside or Pluronic P123), lipid-like (1,2-diheptanoyl- s n-glycero-3-phosphocholine), and perfluoro-octanoate surfactants under mild acidic conditions to coassemble the light-responsive transmembrane protein proteorhodopsin at concentrations up to 15 wt % into the hydrophobic regions of worm-like mesostructured silica materials in films. Small-angle X-ray scattering, electron paramagnetic resonance spectroscopy, and transient UV-visible spectroscopy analyses established that proteorhodopsin molecules in mesostructured silica films exhibited native-like function, as well as enhanced thermal stability compared to surfactant or lipid environments. The light absorbance properties and light-activated conformational changes of proteorhodopsin guests in mesostructured silica films are consistent with those associated with the native H+-pumping mechanism of these biomolecules. The synthetic protocol is expected to be general, as demonstrated also for the incorporation of functionally active cytochrome c, a peripheral membrane protein enzyme involved in electron transport, into mesostructured silica-cationic surfactant films.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP7631869-A	Silica, mesostructured		7631-86-9	Price

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