Blu11 (BlueAQUA) Prestained Protein Ladder

Catalog numberPMB11-0500 / PM019-0500

Size500 μl

Range10 to 180 kDa

UseAs molecular weight standards for SDS-PAGE electrophoresis

Sample25 μl ($7.2)

$132 Inquire

Note: Protein Marker / Protein Standard / SDS-PAGE / Western Blot

Description

The Blu11 (BlueAQUA) Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 10 to 180 kDa. The 11 recombinant proteins are covalently coupled with blue chromophore, and 2 reference bands at 25 kDa and 72 kDa serve as reference bands. The Blu11 (BlueAQUA) Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Properties

Appearance: Liquid
Color: Blue
Odor: Odorless
Weak pH-value at 25 °C: ~7.5
Boiling point: ~ 100 °C

Reaction Setup

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Guide for Molecular Weight Estimation (kDa)

Refer to the Blu11 (BlueAQUA) Prestained Protein Ladder patterns in various electrophoresis conditions :

Blu11 (BlueAQUA) Prestained Protein Ladder

Important notes: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Storage Buﬀer

Approximately 0.1~0.5 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25 °C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

Storage Conditions

Stable for up to 2 weeks at 25 °C.
Stable for up to 3 months at 4 °C.
Stable for up to 24 months at -20 °C.

Required Materials but Not Provided

Vertical Electrophoresis system
Power supplies
Vortex or equivalent
Microcentrifuge