Blu13 (BLUelf) Prestained Protein Ladder

Reaction Setup

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Guide for Molecular Weight Estimation (kDa)

Refer to the Blu13 (BLUelf) Prestained Protein Ladder patterns in various electrophoresis conditions:

Storage Buffer

Approximately 0.1~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25 °C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

• Stable for up to 2 weeks at 25 °C.
• Stable for up to 3 months at 4 °C.
• Stable for up to 24 months at -20 °C.

• Vertical Electrophoresis system
• Power supplies
• Vortex or equivalent
• Microcentrifuge