IRIS9 Prestained Protein Ladder

IRIS9 - Prestained Protein Ladder

Catalog numberPM009-0500

Size500 μl

Range15 to 180 kDa

UseAs molecular weight standards for SDS-PAGE electrophoresis.

$96 Inquire

Note: Protein Marker / Protein Standard / SDS-PAGE / Western Blot / 9 bands / SDS-polyacrylamide gel electrophoresis

Description

The IRIS9 Prestained Protein Ladder is a combination of 9 pre-stained proteins with molecular weights from 15 to 180 kDa. The 9 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 30 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS9 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Properties

Appearance: Liquid
Color: Blue
Odor: Odorless
Weak pH-value at 25 °C: ~7.5
Boiling point: ~100 °C

Guide for Molecular Weight Estimation (kDa)

Refer to the IRIS9 Prestained Protein Ladder patterns in various electrophoresis conditions:

Reaction Setup

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel,
respectively. 2.5 μl per well for general Western transferring

IRIS9 Prestained Protein Ladder

Important notes: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment

Storage Buﬀer

0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

Quality Control

5 μl of IRIS9 Prestained Protein Ladder (PM009) resolves 9 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.

Storage Conditions

Stable for up to 2 weeks at 25 °C.
Stable for up to 3 months at 4 °C.
Stable for up to 24 months at -20 °C.

Required Materials but Not Provided

Vertical Electrophoresis system
Power supplies
Vortex or equivalent
Microcentrifuge