β-Casein from bovine milk

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For Research Use Only | Not For Clinical Use

CAT	AP9000719-D
CAS	9000-71-9
MDL Number	MFCD00130619
EC Number	232-555-1

Description	BioUltra, ≥98% (PAGE)
Assay	≥98% (PAGE)
Form	essentially salt-free, lyophilized powder
Size	250MG, 1G
Storage Conditions	−20°C

A Tryptic Hydrolysate From Bovine Milk αs1-casein Enhances Pentobarbital-Induced Sleep in Mice via the GABAA Receptor

Irene Joy I Dela Peña, Hee Jin Kim, June Bryan de la Peña, Mikyung Kim, Chrislean Jun Botanas, Kyung Yi You, Taeseon Woo, Yong Soo Lee, Jae-Chul Jung, Kyung-Mi Kim, Jae Hoon Cheong

Behav Brain Res. 2016 Oct 15;313:184-190.

PMID: 27401107

Acidification Effects on Isolation of Extracellular Vesicles From Bovine Milk

Md Matiur Rahman, Kaori Shimizu, Marika Yamauchi, Hiroshi Takase, Shinya Ugawa, Ayaka Okada, Yasuo Inoshima

PLoS One. 2019 Sep 16;14(9):e0222613.

PMID: 31525238

Efficient Method for Isolation of Exosomes From Raw Bovine Milk

Marika Yamauchi, Kaori Shimizu, Matiur Rahman, Hinata Ishikawa, Hiroshi Takase, Shinya Ugawa, Ayaka Okada, Yasuo Inoshima

Drug Dev Ind Pharm. 2019 Mar;45(3):359-364.

PMID: 30366501

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Case Study

Preparation of Naringenin-Loaded β-Casein Micelles

Li, Mo, et al. Food Hydrocolloids 96 (2019): 653-662.

Bovine milk protein β-casein (β-CN) is an intrinsically disordered amphiphilic protein that can self-assemble into micelles. Naringenin, a major hydrophobic flavanone found in grapefruit, exhibits various beneficial biological effects such as antioxidant, anticancer, and anti-inflammatory activities. This study demonstrates that naringenin can be encapsulated in β-CN micelles.
Preparation of naringenin-loaded β-CN micelles: β-CN solutions of different concentrations (1, 2, 3, 4, and 5 mg/mL) were prepared in 20 mM sodium phosphate buffer (pH 7.0). Naringenin stock solution (0.168 M in ethanol) was added to the protein solution under magnetic stirring for 2 hours, resulting in a final naringenin/β-CN molar ratio of 12:1. Centrifugation (13,000 rpm, 15 minutes) did not cause naringenin precipitation, indicating complete dissolution of naringenin. The supernatant was stored at 4 °C for further analysis.

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