Cathepsin D/E Substrate, Fluorogenic - Analytical Products / Alfa Chemistry

CAT	IAR42415988
Molecular Weight	1756.01

Description	An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E, but not cathepsins B, H, or L .
Solubility	DMSO: 5 mg/mL; λem ~393 nm
Assay	≥97% (HPLC)
Color	yellow
Form	lyophilized solid; desiccated; protect from light
Size	1MG
Storage Conditions	+2C to +8C

1

Cathepsin D, but Not Cathepsin E, Degrades Desmosomes During Epidermal Desquamation

S Igarashi, T Takizawa, T Takizawa, Y Yasuda, H Uchiwa, S Hayashi, H Brysk, J M Robinson, K Yamamoto, M M Brysk, T Horikoshi

Br J Dermatol. 2004 Aug;151(2):355-61.

PMID: 15327542

1

Characterization of New Fluorogenic Substrates for the Rapid and Sensitive Assay of Cathepsin E and Cathepsin D

Y Yasuda, T Kageyama, A Akamine, M Shibata, E Kominami, Y Uchiyama, K Yamamoto

J Biochem. 1999 Jun;125(6):1137-43.

PMID: 10348917

1

Proteolytic Activity of Human Osteoclast Cathepsin K. Expression, Purification, Activation, and Substrate Identification

M J Bossard, T A Tomaszek, S K Thompson, B Y Amegadzie, C R Hanning, C Jones, J T Kurdyla, D E McNulty, F H Drake, M Gowen, M A Levy

J Biol Chem. 1996 May 24;271(21):12517-24.

PMID: 8647860

1

Purification and Characterization of Recombinant Human Cathepsin E Expressed in Human Kidney Cell Line 293

Maria G Cappiello, Zhongren Wu, Boyd B Scott, Gerard M McGeehan, Richard K Harrison

Protein Expr Purif. 2004 Sep;37(1):53-60.

PMID: 15294281

1

Selective Detection of Cathepsin E Proteolytic Activity

Wael R Abd-Elgaliel, Ching-Hsuan Tung

Biochim Biophys Acta. 2010 Sep;1800(9):1002-8.

PMID: 20600629