Hygromycin B from Streptomyces hygroscopicus

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For Research Use Only | Not For Clinical Use

CAT	AP31282049
CAS	31282-04-9
Structure
MDL Number	MFCD06795479
Molecular Weight	527.52
EC Number	250-545-5
InChI Key	GRRNUXAQVGOGFE-XKIAHZFYSA-N

Description	powder, BioReagent, suitable for cell culture, suitable for insect cell culture
Solubility	H2O: 50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 30 days.)
Assay	≥60% (HPLC)
Color	faintly brown to brown; white to beige; (1) 7.1, (2) 8.8
Form	powder; cell culture \| mammalian: suitable
MP	160-180 °C
Size	50MG, 100MG, 250MG, 5X100MG, 1G
Storage Conditions	2-8°C

Directed Evolution for Thermostabilization of a Hygromycin B Phosphotransferase From Streptomyces Hygroscopicus

Naohisa Sugimoto, Yasuaki Takakura, Kentaro Shiraki, Shinya Honda, Naoki Takaya, Takayuki Hoshino, Akira Nakamura

Biosci Biotechnol Biochem. 2013;77(11):2234-41.

PMID: 24200799

Molecular Cloning and Expression in Streptomyces Lividans of a Hygromycin B Phosphotransferase Gene From Streptomyces Hygroscopicus

F Malpartida, M Zalacaín, A Jiménez, J Davies

Biochem Biophys Res Commun. 1983 Nov 30;117(1):6-12.

PMID: 6318753

Nucleotide Sequence of the Hygromycin B Phosphotransferase Gene From Streptomyces Hygroscopicus

M Zalacain, A González, M C Guerrero, R J Mattaliano, F Malpartida, A Jiménez

Nucleic Acids Res. 1986 Feb 25;14(4):1565-81.

PMID: 3005976

Purification and Characterization of a Hygromycin B Phosphotransferase From Streptomyces Hygroscopicus

M Zalacain, J M Pardo, A Jiménez

Eur J Biochem. 1987 Jan 15;162(2):419-22.

PMID: 3026811

Structural Basis for the Substrate Recognition of Aminoglycoside 7''-phosphotransferase-Ia From Streptomyces Hygroscopicus

Mihoko Takenoya, Tatsuro Shimamura, Ryuji Yamanaka, Yuya Adachi, Shinsaku Ito, Yasuyuki Sasaki, Akira Nakamura, Shunsuke Yajima

Acta Crystallogr F Struct Biol Commun. 2019 Sep 1;75(Pt 9):599-607.

PMID: 31475927

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