CAS 70288-86-7 Ivermectin - Analytical Products / Alfa Chemistry

CAT	APB70288867
CAS	70288-86-7
Structure
Molecular Weight	875.11
Molecular Formula	C48H74O14

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Case Study

Ivermectin as an Internal Standard for High-Sensitivity Pharmacokinetic Studies of Moxidectin in Human Plasma

Kitzman, Dennis, She-Yi Wei, and Lawrence Fleckenstein. Journal of pharmaceutical and biomedical analysis 40.4 (2006): 1013-1020.

Ivermectin has been effectively utilized as an internal standard in high-performance liquid chromatography (HPLC) methods for quantifying moxidectin in human plasma. Due to moxidectin's extensive volume of distribution, an ultrasensitive detection method is required for pharmacokinetic studies. The developed HPLC-fluorescence assay, incorporating ivermectin as an internal standard, enabled the detection of multiple unidentified metabolite peaks post-dosing, highlighting its superior sensitivity compared to previously reported methods.
Stock solutions of ivermectin and moxidectin were prepared in acetonitrile at a concentration of 1.0 mg/mL, followed by serial dilutions for calibration curve preparation. A 40 ng/mL ivermectin solution was selected as the internal standard, achieving a peak height ratio of 1.0 at the midpoint of the moxidectin standard curve. The separation was optimized using an Ultrasphere C18 column, improving peak resolution and shape. Solid-phase extraction and fluorescence detection were employed to enhance sensitivity.
The developed HPLC method demonstrated high specificity and sensitivity, with ivermectin providing a stable and reproducible internal standard signal. Unexpectedly, multiple unidentified metabolite peaks of moxidectin appeared in plasma samples post-administration, which were not previously reported in other methods. The extended chromatographic run time (32 min) was advantageous in distinguishing these metabolites.

Ivermectin Reference Standard for the Development and Validation of Chromatographic and Spectrophotometric Assays in Pharmaceutical Preparations

Taşpınar, Numan. "Comparation with spectrophotometric and liquid chromatographic methods of pharmaceutical forms of Ivermectin." Medical Records 5.1 (2023): 126-31.

Ivermectin reference standard is pivotal for developing and validating reliable analytical methods for the quantification of ivermectin in pharmaceutical formulations. This case study explores the use of ivermectin reference standard in liquid chromatography (LC) and ultraviolet (UV) spectrophotometry for assessing ivermectin content in tablets and injectable solutions.
Ivermectin, a semi-synthetic antiparasitic agent, requires precise analytical methods for quality control and pharmaceutical analysis. In this study, we developed liquid chromatographic and UV spectrophotometric methods, employing ivermectin reference standard for calibration and validation.
Ivermectin reference standard was dissolved in methanol to prepare stock solutions, which were further diluted to obtain standard concentrations. Chromatographic separation was performed using an Agilent C18 column with a mobile phase consisting of acetonitrile and ultrapure water at a 1.2 mL/min flow rate, and detection was done at 245 nm. The UV spectrophotometric analysis also utilized the same wavelength for absorbance measurement.
Both methods were validated according to ICH Q2(R1) guidelines, demonstrating precision, accuracy, and linearity with recovery rates between 98-100%. Statistical analysis showed no significant difference between the two methods. Both LC and UV methods proved suitable for determining ivermectin content in pharmaceutical products.

Ivermectin Standard for Mass Spectrometric Analysis and Tissue Concentration Estimation in Canine Samples

Lehner, A. F., et al. Journal of Mass Spectrometry 44.1 (2009): 111-119.

Ivermectin standard plays a crucial role in the accurate identification and quantification of ivermectin in biological samples. This case study highlights the use of ivermectin standard in electrospray ionization mass spectrometry (ESI-MS) and tandem mass spectrometry (ESI-MS-MS) for determining ivermectin concentrations in canine tissue extracts.
Ivermectin, a macrolide parasiticide, exists primarily as two analogs: B1a (80%) and B1b (20%). The ivermectin standard was analyzed by ESI-MS, revealing a principal peak at m/z 897 due to sodium ion complexation, rather than the expected m/z 875 for the B1a analog. This study focuses on the application of ivermectin standard for identifying its presence in canine tissue samples.
The ivermectin standard was analyzed using direct infusion-ESI-MS-MS. Canine tissue extracts were prepared through simple ethyl alcohol extraction, and mass spectra were compared with the ivermectin standard. This enabled the estimation of ivermectin concentrations in the tissues, with results of 20 µg/g for case C998-06 and 40 µg/g for case C999-06.
The ESI-MS-MS method provided reliable, fast, and sensitive semi-quantitative results. The mass spectral peaks from canine samples matched those of the ivermectin standard, confirming its administration and estimating tissue concentrations.