CAS 521-31-3 Luminol - Analytical Products / Alfa Chemistry

CAT	AP521313
CAS	521-31-3
Structure
MDL Number	MFCD00006890
Molecular Weight	177.16
EC Number	208-309-4
InChI Key	HWYHZTIRURJOHG-UHFFFAOYSA-N
REAXYS Number	383929

Description	≥97% (HPLC)
Assay	≥97% (HPLC)
MP	>300 °C (lit.)
Size	5G, 25G

[Development and Application of Catalytic Tyrosine Modification]

Shinichi Sato, Michihiko Tsushima, Kosuke Nakamura, Hiroyuki Nakamura

Yakugaku Zasshi. 2018;138(1):39-46.

PMID: 29311464

Luminol, Horseradish Peroxidase, and Glucose Oxidase Ternary Functionalized Graphene Oxide for Ultrasensitive Glucose Sensing

Fang Li, Wenjing Ma, Jiachang Liu, Xiang Wu, Yan Wang, Jianbo He

Anal Bioanal Chem. 2018 Jan;410(2):543-552.

PMID: 29167935

Peroxidase-catalyzed Chemiluminescence System and Its Application in Immunoassay

Zhao Zhang, Jiahui Lai, Kesen Wu, Xingcan Huang, Shuai Guo, Lili Zhang, Jian Liu

Talanta. 2018 Apr 1;180:260-270.

PMID: 29332809

Self-Catalyzing Chemiluminescence of Luminol-Diazonium Ion and Its Application for Catalyst-Free Hydrogen Peroxide Detection and Rat Arthritis Imaging

Chunxin Zhao, Hongbo Cui, Jing Duan, Shenghai Zhang, Jiagen Lv

Anal Chem. 2018 Feb 6;90(3):2201-2209.

PMID: 29285924

Sensitive determination of bromhexine hydrochloride based on its quenching effect on luminol/H 2 O 2 electrochemiluminescence system

Dexian Kong, Siqin Huang, Jiansong Cheng, Qizhao Zhuang, Yijun Liu, Chun-Hua Lu

Luminescence. 2018 Jun;33(4):698-703.

PMID: 29605968

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Case Study

Luminol Used for the Construction of a Ni-MOF@Pt-Based ECL Sensor for Oxytetracycline Detection

Tian, Li, et al. Food Chemistry 482 (2025): 144019.

Luminol, a classic chemiluminescent reagent, has been effectively utilized for constructing a novel electrochemiluminescence (ECL) sensor for the ultrasensitive detection of oxytetracycline (OTC). In this study, luminol was incorporated into β-cyclodextrin (β-CD) to form a high-capacity luminol@β-CD complex, enhancing its luminescent efficiency. Simultaneously, platinum nanoparticles (Pt NPs), known for their electron transfer enhancement, were immobilized onto nickel-based metal-organic frameworks (Ni-MOF) to generate Ni-MOF@Pt NPs, functioning as co-reaction accelerators in the luminol-O₂ system.
The sensor was fabricated by sequential modification of a polished glassy carbon electrode (GCE). First, 4 μL of luminol@β-CD solution was cast onto the GCE and dried, followed by 4 μL of Ni-MOF@Pt NPs solution. A final coating with 5 μL of 0.05% Nafion ensured film stability. This layered Ni-MOF@Pt NPs/luminol@β-CD/Nafion/GCE architecture yielded significantly enhanced luminescence due to the synergistic contributions of luminol and Pt NPs.
OTC detection was achieved via a quenching mechanism based on energy transfer, with luminol acting as the donor and OTC as the acceptor. The sensor demonstrated high sensitivity and selectivity, offering a robust platform for antibiotic residue monitoring.
This work highlights luminol's pivotal role in ECL sensing applications, especially when combined with MOF-based nanocomposites, underscoring its value in environmental and pharmaceutical analyses.

Luminol Used for the Preparation of Electrochemiluminescent Nanoprobes in Point-of-Care cTnI Detection

Chen, Jun, et al. Talanta 293 (2025): 128159.

Luminol, a widely employed chemiluminescent reagent, has been successfully utilized in the preparation of luminol-labeled silver nanoparticles (luminol@AgNPs) for constructing an electrochemiluminescence lateral flow immunosensor (ECL-LFI) targeting cardiac troponin I (cTnI). As cTnI is the gold-standard biomarker for acute myocardial infarction (AMI) diagnosis, its detection at trace levels is crucial for early intervention.
In this study, luminol@AgNPs were synthesized by introducing luminol into a silver nitrate solution under alkaline conditions, followed by nanoparticle isolation through centrifugation. The nanoparticles were further conjugated with anti-cTnI antibodies via electrostatic adsorption to create specific immune nanoprobes. These functionalized particles were then incorporated into an ECL-LFI platform comprising a conjugate pad, nitrocellulose membrane, absorbent pad, and a screen-printed electrode (SPE).
Upon the addition of a cTnI-containing sample and luminol-H₂O₂ co-reactant, a sandwich immunocomplex was formed on the strip, producing a quantifiable ECL signal. The system demonstrated an impressive linear detection range of 5 pg/mL to 100 ng/mL and a detection limit of 1.6 pg/mL-ideal for early AMI diagnostics.
This case exemplifies luminol's utility in advanced biosensing applications, particularly in enhancing signal sensitivity and specificity for point-of-care diagnostics through nanomaterial-based ECL systems.

Luminol Used for the Preparation of a Dual-Potential Ratiometric Electrochemiluminescence Sensor for Kanamycin Detection

Li, Chao, et al. Food Chemistry 479 (2025): 143840.

Luminol, a classic chemiluminescent reagent, has been effectively utilized in constructing a novel dual-potential ratiometric electrochemiluminescence (RECL) sensor for the ultrasensitive detection of kanamycin (KAN). In this system, luminol is anchored onto a Ti₃CN nanosheet support (Ti₃CN@luminol), enhancing anodic ECL signals and providing high signal stability.
The RECL sensor design involves integrating luminol at the anode and porous graphite-phase carbon nitride (PCN) at the cathode. PCN serves dual roles as a cathodic luminescent material and a carrier for Pt-Au nanoparticles (NPs), thereby amplifying cathodic ECL emission. The Ti₃CN@luminol composite, prepared via ultrasonic dispersion of luminol in NaOH with Ti₃CN, exhibits strong ECL activity and was coated onto a Pt-Au NPs/PCN-modified glassy carbon electrode (GCE), with chitosan added as a stabilizing top layer.
This sensor demonstrates exceptional sensitivity, with a linear response between the ECL signal ratio (I_luminol/I_PCN) and the logarithm of KAN concentration from 5.0 × 10⁻¹⁴ to 5.0 × 10⁻⁸ mol/L, and an ultra-low detection limit of 1.67 × 10⁻¹⁴ mol/L. The system has been validated for KAN detection in milk samples.
This case highlights the critical role of luminol in advanced ratiometric sensing platforms, providing a promising approach for trace antibiotic residue monitoring in food safety analysis.