CAS 1431724-30-9 PACA - Analytical Products / Alfa Chemistry

CAT	AP1431724309
CAS	1431724-30-9
Molecular Weight	217.22
InChI Key	HLHSUNWAPXINQU-GQCTYLIASA-N

Description	≥98% (HPLC)
Solubility	DMSO: 25 mg/mL, clear
Assay	≥98% (HPLC)
Color	white to light brown
Form	powder
Size	5MG, 25MG
Storage Conditions	2-8°C

Chemoresistance Transmission via Exosome-Mediated EphA2 Transfer in Pancreatic Cancer

Jia Fan, Qian Wei, Eugene J Koay, Yang Liu, Bo Ning, Paul W Bernard, Ning Zhang, Haiyong Han, Matthew H Katz, Zhen Zhao, Ye Hu

Theranostics. 2018 Nov 13;8(21):5986-5994.

PMID: 30613276

Combined Evaluation of a Panel of Protein and miRNA Serum-Exosome Biomarkers for Pancreatic Cancer Diagnosis Increases Sensitivity and Specificity

Bindhu Madhavan, Shijing Yue, Uwe Galli, Sanyukta Rana, Wolfgang Gross, Miryam Müller, Nathalia A Giese, Holger Kalthoff, Thomas Becker, Markus W Büchler, Margot Zöller

Int J Cancer. 2015 Jun 1;136(11):2616-27.

PMID: 25388097

Ping-Pong-Tumor and Host in Pancreatic Cancer Progression

Wei Mu, Zhe Wang, Margot Zöller

Front Oncol. 2019 Dec 16;9:1359.

PMID: 31921628

Re-designing Interleukin-12 to Enhance Its Safety and Potential as an Anti-Tumor Immunotherapeutic Agent

Pengju Wang, Xiaozhu Li, Jiwei Wang, Dongling Gao, Yuenan Li, Haoze Li, Yongchao Chu, Zhongxian Zhang, Hongtao Liu, Guozhong Jiang, Zhenguo Cheng, Shengdian Wang, Jianzeng Dong, Baisui Feng, Louisa S Chard, etc.

Nat Commun. 2017 Nov 9;8(1):1395.

PMID: 29123084

The Effect of GPRC5a on the Proliferation, Migration Ability, Chemotherapy Resistance, and Phosphorylation of GSK-3β in Pancreatic Cancer

Bin Liu, Hai Yang, Christian Pilarsky, Georg F Weber

Int J Mol Sci. 2018 Jun 26;19(7):1870.

PMID: 29949874

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Case Study

N-propargyl caffeamide (PACA) Enhances Conversion of proNGF to NGF in Midbrain Tissues

Luo, Dan, et al. Molecular neurobiology 55 (2018): 2258-2267.

To determine the effect of PACA on NGF and proNGF protein levels in mouse brain, NGF and proNGF protein levels were determined by Western blotting using specific antibodies. In mice treated with MPTP, NGF protein levels in SNpc were reduced. PACA effectively restores NGF protein levels to resist the neurotoxicity of MPTP. MPTP greatly increased the accumulation of proNGF, while PACA reduced proNGF protein levels in the midbrain of mice treated with MPTP.
In order to verify the effect of PACA on NGF and proNGF protein levels, we further measured NGF levels through immunofluorescent staining. Midbrain tissue was recovered from mice after treatment with a combination of vehicle, MPTP, MPTP and PACA. MPTP almost eliminated binding of anti-NGF antibodies, while PACA restored retention of anti-NGF antibodies in the midbrain of MPTP-treated mice. Based on immunostaining of NGF and GFAP in the substantia nigra, a dose of 15 mg/kg/day PACA increased NGF levels not only in astrocytes, but also in other cell types. These results suggest that PACA increases the conversion of proNGF to NGF in midbrain tissue.

N-Propargyl Caffeate Amide (PACA) Enhances NGF-Induced Neuritogenesis

Yang, C., Zhao, J., Cheng, Y., Le, X. C., & Rong, J. (2015). ACS Chemical Neuroscience, 6(9), 1560-1569.

To evaluate the neuritogenic activity of PACA, PC12 cells were treated with NGF (2 ng/mL) alone or in combination with PACA at concentrations of 5, 10, and 20 μM for 72 hours. Neurite outgrowth was then analyzed by staining the cells with a neurite outgrowth staining kit and examining them under a fluorescence microscope to identify neurites longer than 20 μm. Compared to NGF alone, PACA significantly enhanced NGF-induced neuritogenesis in a concentration-dependent manner. Notably, PACA alone at a concentration of 20 μM did not induce neuritogenesis. Interestingly, the combination of PACA (20 μM) and NGF (2 ng/mL) resulted in an approximately six-fold increase in the number of neurite-bearing cells compared to NGF alone. After treating PC12 cells with PACA and NGF, either alone or in combination, the expression of neuronal biomarkers such as β3-tubulin, MAP2, and GAP-43 was analyzed by Western blotting. PACA significantly enhanced NGF's activity in inducing these neuronal markers. In conclusion, these results suggest that PACA can potentiate NGF's neurogenic activity in terms of neurite extension.

N-Propargyl Caffeamide (PACA) Protects the Heart from Ischemia-Induced Cardiac Injury

Cheng, Yuanyuan, et al. Cellular Physiology and Biochemistry 47.6 (2018): 2544-2557.

To evaluate the cardioprotective effects of PACA in vivo, myocardial infarction was induced in rats by ligating the left anterior descending (LAD) artery. PACA was administered via intraperitoneal injection 24 hours after arterial ligation. According to TTC staining, PACA reduced the myocardial infarction area from 34.5% to 15.0% by the third day. H&E staining revealed the destruction of myofibril structure and the accumulation of neutrophil/macrophage clusters in the ischemic myocardium. PACA at a dose of 15 mg/kg not only preserved myofibril structure but also reduced the recruitment of inflammatory cells and myolysis.