Combined Use of Ionic Liquid and hydroxypropyl-β-cyclodextrin for the Enantioseparation of Ten Drugs by Capillary Electrophoresis

Yan Cui, Xiaowei Ma, Min Zhao, Zhen Jiang, Shuying Xu, Xingjie Guo

Chirality. 2013 Jul;25(7):409-14.

PMID: 23740623

Abstract:

In the present study, hydroxypropyl-β-cyclodextrin and an ionic liquid (1-ethyl-3-methylimidazolium-l-lactate) were used as additives in capillary electrophoresis for the enantioseparation of 10 analytes, including ofloxacin, propranolol hydrochloride, dioxopromethazine hydrochloride, isoprenaline hydrochloride, chlorpheniramine maleate, liarozole, tropicamide, amlodipine benzenesulfonate, brompheniramine maleate, and homatropine methylbromide. The effects of ionic liquid concentrations, salt effect, cations, and anions of ionic liquids on enantioseparation were investigated and the results proved that there was a synergistic effect between hydroxypropyl-β-cyclodextrin and the ionic liquid, and the cationic part of the ionic liquid played an important role in the increased resolution. With the developed dual system, all the enantiomers of 10 analytes were well separated in resolutions of 5.35, 1.76, 1.85, 2.48, 2.88, 1.43, 5.45, 4.35, 2.76, and 2.98, respectively. In addition, the proposed method was applied to the determination of the enantiomeric purity of S-ofloxacin after validation of the method in terms of selectivity, repeatability, linearity range, accuracy, precision, limit of detection (LOD), and limit of quality (LOQ).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP2438326-B	S-(+)-Chlorpheniramine maleate salt		2438-32-6	Price
AP878132195	1-Ethyl-3-methylimidazolium L-(+)-lactate		878132-19-5	Price