Determination of the Composition, Encapsulation Efficiency and Loading Capacity in Protein Drug Delivery Systems Using Circular Dichroism Spectroscopy

Zhili Peng, Shanghao Li, Xu Han, Abdulrahman O Al-Youbi, Abdulaziz S Bashammakh, Mohammad S El-Shahawi, Roger M Leblanc

Anal Chim Acta. 2016 Sep 21;937:113-8.

PMID: 27590552

Abstract:

Peptides and proteins have become very promising drug candidates in recent decades due to their unique properties. However, the application of these drugs has been limited by their high enzymatic susceptibility, low membrane permeability and poor bioavailability when administered orally. Considerable efforts have been made to design and develop drug delivery systems that could transport peptides and proteins to targeted area. Although it is of great importance to determine the composition after loading a drug to the carrier, the ability to do so is significantly limited by current analytical methods. In this letter, five important proteins, α1-antitrypsin, hemoglobin human, human serum albumin, human transferrin and r-globulin were chemically conjugated to two model drug carriers, namely carbon dots and polymer O-(2-carboxyethyl) polyethylene glycol. A simple yet convenient method based on circular dichroism spectroscopy was developed to determine the compositions of the various protein-carrier conjugates.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP117786944	O-(2-Carboxyethyl)polyethylene glycol		117786-94-4	Price
LS7411195	O-(2-Carboxyethyl)-O′-methyl-undecaethylene glycol			Price