Efficient Production of 2,3-butanediol in Saccharomyces Cerevisiae by Eliminating Ethanol and Glycerol Production and Redox Rebalancing

Sujin Kim, Ji-Sook Hahn

Metab Eng. 2015 Sep;31:94-101.

PMID: 26226562

Abstract:

2,3-Butanediol is a promising valuable chemical that can be used in various areas as a liquid fuel and a platform chemical. Here, 2,3-butanediol production in Saccharomyces cerevisiae was improved stepwise by eliminating byproduct formation and redox rebalancing. By introducing heterologous 2,3-butanediol biosynthetic pathway and deleting competing pathways producing ethanol and glycerol, metabolic flux was successfully redirected to 2,3-butanediol. In addition, the resulting redox cofactor imbalance was restored by overexpressing water-forming NADH oxidase (NoxE) from Lactococcus lactis. In a flask fed-batch fermentation with optimized conditions, the engineered adh1Δadh2Δadh3Δadh4Δadh5Δgpd1Δgpd2Δ strain overexpressing Bacillus subtilis α-acetolactate synthase (AlsS) and α-acetolactate decarboxylase (AlsD), S. cerevisiae 2,3-butanediol dehydrogenase (Bdh1), and L. lactis NoxE from a single multigene-expression vector produced 72.9 g/L 2,3-butanediol with the highest yield (0.41 g/g glucose) and productivity (1.43 g/(L · h)) ever reported in S. cerevisiae.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP19132060	L-(+)-2,3-Butanediol		19132-06-0	Price