NT-3-secreting Human Umbilical Cord Mesenchymal Stromal Cell Transplantation for the Treatment of Acute Spinal Cord Injury in Rats

Ai-Jia Shang, Sun-Quan Hong, Qiang Xu, Hang-Yan Wang, Ying Yang, Zhong-Feng Wang, Bai-Nan Xu, Xiao-Dan Jiang, Ru-Xiang Xu

Brain Res. 2011 May 19;1391:102-13.

PMID: 21420392

Abstract:

An animal model for clip spinal cord injury (SCI) was used to determine whether Neurotrophin-3 (NT-3) genetically modified human umbilical mesenchymal stem cells (NT-3-HUMSCs) could promote the morphologic and functional recovery of injured spinal cords. Using the Basso, Beattie, and Bresnahan scores and a grid test, the rats in the HUMSC-treated and NT-3-HUMSCs groups had significantly improved locomotor functional recovery more than the control group. In comparison, the NT-3-HUMSCs group achieved better functional recovery than the HUMSCs group at the end of 12 weeks after SCI. The functional recovery was accompanied by increased intensity of 5-HT fibers, increased volume of spared myelination, and decreased area of the cystic cavity in the NT-3-HUMSCs group compared with the HUMSCs group. Moreover, transplanted NT-3-HUMSCs survived and produced larger amounts of NT-3 than the HUMSCs in the host spinal cord. These results show that NT-3 enhanced the therapeutic effects of HUMSCs after clip injury of the spinal cord.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248821	NT-3 human			Price