Purification by Silica Gel Chromatography Using Dialysis Tubing and Characterization of Sophorolipids Produced From Candida Bombicola Grown on Glucose and Arachidonic Acid

Sachin Shah, Asmita Prabhune

Biotechnol Lett. 2007 Feb;29(2):267-72.

PMID: 17091375

Abstract:

The yeast Candida bombicola (ATCC 22214) grown on primary carbon source glucose (100 g l(-1)) and secondary carbon, arachidonic acid (2 g l(-1)) produced mixture of sophorolipids up to 1.44 g l(-1). The crude product was a heterogeneous mixture of sophorolipids, which are glycolipids of sophorose linked to the fatty acid through glycosidic bond between omega and omega-1 carbon of arachidonic acid. The derived sophorolipids were isolated by silica gel chromatography using dialysis tubing. The purified sophorolipids were characterized by ESI-MS and FT-IR. Acid hydrolysis of the resolved sophorolipids were characterized by ESI-MS for the presence of 20-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid (20-HETE) and 19-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid (19-HETE), compounds of pronounced pharmaceutical importance.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP79551863	20-Hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoic acid		79551-86-3	Price