Rapid Identification of New Bacterial Alcohol Dehydrogenases for (R)- And (S)-enantioselective Reduction of Ss-Ketoesters

Jie Zhang, Wouter A Duetz, Bernard Witholt, Zhi Li

Chem Commun (Camb). 2004 Sep 21;(18):2120-1.

PMID: 15368004

Abstract:

New bacterial alcohol dehydrogenases with high and complementary enantioselectivity for the reduction of ethyl 3-keto-4,4,4-trifluorobutyrate 1 and methyl 3-keto-3-(3'-pyridyl)-propionate 3 have been rapidly identified by use of a new methodology consisting of preselection of microorganisms based on degradation ability and high-throughput screening with a miniaturized system coupled with fast analysis of enantioselectivities.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP371266	Ethyl 4,4,4-trifluorobutyrate		371-26-6	Price