Comparative Study of Deoxynivalenol, 3-acetyldeoxynivalenol, and 15-acetyldeoxynivalenol on Intestinal Transport and IL-8 Secretion in the Human Cell Line Caco-2

Tomoyuki Kadota, Hiroko Furusawa, Satoshi Hirano, Osamu Tajima, Yoichi Kamata, Yoshiko Sugita-Konishi

Toxicol In Vitro. 2013 Sep;27(6):1888-95.

PMID: 23792671

Abstract:

The effects of the trichothecene mycotoxin deoxynivalenol (DON) and its acetylated derivatives, 3-acetyldeoxynivalenol (3ADON) and 15-acetyldeoxynivalenol (15ADON) on human intestinal cell Caco-2 were investigated by the studies of transepithelial transport, gene expression, and cytokine secretion. Permeability across a Caco-2 cell monolayer was evaluated by transport study. Transport rates were ranked as DON, 3ADON<15ADON in apical-basolateral direction. 15ADON showed the highest permeability, induced the highest decrease in transepithelial electrical resistance (TEER), and prompted significant Lucifer Yellow permeability. These results showed that 15ADON affect paracellular barrier function extremely. In addition, gene expressions induced by toxins were screened by DNA microarray for investigating cellular effect on Caco-2 cell. The most remarkable gene induced by DON and 15ADON was inflammatory chemokine IL-8 and thus mRNA expression and secretion of IL-8 were analyzed by PCR and ELISA. Both DON and acetylated DONs could induce mRNA expression and production of IL-8. In particular, ELISA assay showed that the ability to produce IL-8 was ranked as 3ADON