Van Gieson Stain

Van Gieson Stain

Assay-kits

Product code: ACMBS-27
Specifications: 3x20mL, 3x100mL (Box)
Intended use: For differential staining of tissue cytology collagen fibers and muscle fibers
Store temperature: 5℃～30℃
Expiry date: 18 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationPrecautions

Principle

This method mainly uses acid fuchsin and picric acid to double dye the collagen fiber and the muscle fiber respectively. The bitter acid has a small molecular weight, can penetrate muscle fibers, glial cells and red blood cells with dense tissue structure combining with bitter acid were stained in yellow, The acid fuchsin has a medium to large molecular weight, and can only penetrate collagen fibers with looser tissue structure. combining with acid fuchsin were stained in to red to pink.

Main components

Reagent composition	Main ingredient
1. Weigert's iron hematoxylin solution A	Hematoxylin
2. Weigert's iron hematoxylin solution B	Ferric chloride
3. Van Gieson stain	Ferric chloride

Sample requirement

The tissue section must be fully fixed.

Test procedure

1. The slices are routinely dewaxed to water and washed once with distilled water.
2. Add Weigert's Iron Hematoxylin Solution A, and then add Weigert's Iron Hematoxylin Solution B mixed with it (A: B = 1:1), staining for 5～10 minutes, slightly washed.
3. Rapidly differentiate with 1% hydrochloric acid alcohol and wash for several minutes.
4. Staining with Van Gieson stain (abbreviation: VG) for 1～2 minutes.
5. After pouring Van Gieson stain, directly differentiated with 95% alcohol for several seconds.
6. Conventional dehydration and transparent, sealed with neutral resin.

Result interpretation

Collagen fibers	Red
Muscle fibers, Nerve glial, Erythrocytes	Yellow
Nuclei	Purple black

Precautions

1. Weigert’s Iron Hematoxylin is divided into two liquids A and B. Should mix with two liquids prior to use. It is not suitable to pre-mix, otherwise it will easily oxidize and precipitate then gradually lose the staining power.
2. Differentiate tissue quickly in 95% ethanol after Van Gieson stain. Otherwise it is easily discolored excessively.
3. Because acid fuchsin is soluble in water, after staining in VG solution do not rinse in water. Instead, add 95% ethanol directly to differentiate and then dehydrate in absolute ethanol. Otherwise, the colors from the VG stain will weaken or discolor.
4. This reagent should be used by professionals and interpretation of the results. Read the instruction manual carefully before use, use it within the validity period, and do personal hygiene protection.
5. Dispose of waste according to the requirements of the hospital or environmental protection department after use.
6. The production date, production batch number and expiration date are shown in the outer packaging.

References

1. Chinese Medical Association. Clinical technical operating specifications-pathology volumes [M]. People's Military Medical Publishing House.

2. Ling Qibo. Practical pathology special staining and histochemistry technology [M]. Guangdong Higher Education Press.

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