Wright Stain

Wright Stain

Assay-kits

Product code: ACMBS-14
Specifications: 3x500mL (Box)
Intended use: This kit is for staining bone marrow cell and blood cell smear
Store temperature: 10℃～30℃
Expiry date: 24 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationMethod limitationPrecautions

Principle

Wright Stain, modified from Romanowsky Stain technique. Staining of cells involves physical adsorption and chemical affinity which allows stain to penetrate and remain within cells. Because each cell and its components are different in chemical composition, their affinity to acid stain (Eosin) and alkaline stain (Methylene blue) of this kit varies significantly. After staining the smear with Wright Stain, different types of cells will appear in different colors. Consequently, one can identify cells based on their stain color, shape and other physical characteristics.

Main components

Reagent composition	Main ingredient
1. Wright solution	Methanol, Wright dye
2. Phosphate buffer (pH 6.8)	Phosphate

Sample requirement

Fresh bone marrow cell smear and blood cell smear.

Test procedure

1. Add Wright solution (about 0.5～0.8 ml) to the smear and allow to cover the whole specimen. Stain for 1 minute.
2. Add Phosphate buffer (pH 6.8) (2～3 times of Wright solution) onto Wright solution and mix thoroughly with a rubber pipette bulb. The blood smear was stained for 6～10 minutes, and the bone marrow smear was stained for 30～40 minutes. (This time is for reference only, and the specific dyeing time should vary depending on the number of cells.)
Rinse with water (Please do not first drain the staining solution, should be flushed by running water, to prevent sediment precipitation in the specimen.), waiting for dryness, microscopic examination.

Result interpretation

RBC appear pink, WBC cytoplasmic granules clear, and shows a variety of cell-specific color, nucleus stained purple-red, clear nuclear chromatin structure

Method limitation

Only use for morphological staining to observe.

Precautions

1. The length of staining depends on specimen type, smear thickness, the number of nucleated cells, cell type and room temperature etc. At lower room temperature, extend the staining time as needed. If the eosinophil appears becomes to base, to consider whether the time is too long staining.
2. For bone marrow smear, as there are many fibrins that will clot quickly, the process of smearing must be fast. Do not use oxalate as anticoagulant, doing so would cause the nucleus to distort, chromatin to become compact, cytoplasm vacuole to form and oxalate crystal to appear.
3. The staining should be performed with a sufficient volume to avoid drying out of the sample, to prevent the dye deposition on the smear.
4. This product should be used by professionals and the results of the interpretation.
5. Before use, please read user's instruction sheets carefully. Used within the validity period. And do personal hygiene protection.
6. After used, the waste should be disposed of according to the requirements of the hospital or the environmental protection department.
7. Tightly cap the reagent bottle immediately after use to avoid evaporation.
8. The production date, production batch number and expiration date on the package.

References

1. The PRC Ministry of Health Medical Administrative Department. National Clinical Laboratory Procedures (M) version 3. Nanjing: Southeast University Press, 2006.

2. Li Yuantang, Li Guiqin, etc. Atlas of Clinical Exfoliated Cytology [M]. Shandong Science and Technology Press.

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