Mast Cell Stain (Aldehyde Fuchsin-Orange G Method)

Mast Cell Stain (Aldehyde Fuchsin-Orange G Method)
Mast Cell Stain (Aldehyde Fuchsin-Orange G Method)

Assay-kits

Mast Cell Stain (Aldehyde Fuchsin-Orange G Method)

  • Product code: ACMBS-31
  • Specifications: 2x20mL (Box)
  • Intended use: Used for staining of histological mast cell
  • Store temperature: 2℃~8℃
  • Expiry date: 12 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationPrecautions

Principle

Aldehyde fuchsin has a strong affinity regarding to the containing sulfate group of mucopolysaccharide material, inside Mast cell granules has containing nucleic acid and carboxyl group of heparins, so easy and aldehyde fuchsin combined into complexes and coloring.

Main components

Reagent compositionMain ingredient
1. Aldehyde fuchsin solutionBasic fuchsin
2. Orange G solutionOrange G

Sample requirement

  • The tissue should be fresh and quickly fixed, and its particles destroyed by the long-term storage of the tissue.

Test procedure

  • 1. The slices are routinely dewaxed to water and washed once with distilled water.
  • 2. Add 75% ethanol wash slightly.
  • 3. Add Aldehyde fuchsin solution staining for 10~15 minutes, 75% ethanol differentiated and wash away excess stain solution, slightly water washed.
  • 4. Orange G solution drop staining about 3~5 seconds, slightly water washed.
  • 5. Conventional dehydration and transparent, sealed with neutral resin.

Result interpretation

Mast cell granulesPurple-red to dark purple
Elastic fibersDeep purple
Red blood cellsFresh orange yellow
The remaining tissueLight yellow

Precautions

  • 1. Mast cells staining require fresh tissue and quickly fixed.
  • 2. This reagent should be used by professionals and interpretation of the results. Read the instruction manual carefully before use, use it within the validity period, and do personal hygiene protection.
  • 3. Dispose of waste according to the requirements of the hospital or environmental protection department after use.
  • 4. The production date, production batch number and expiration date are shown in the outer packaging.

References

1. Chinese Medical Association. Clinical technical operating specifications-pathology volumes [M]. People's Military Medical Publishing House.

2. Ling Qibo. Practical pathology special staining and histochemistry technology [M]. Guangdong Higher Education Press.

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