Bacterial Spore Stain

Assay-kits

Product code: ACMBS-57
Specifications: 3x20mL(Box)
Intended use: Used for bacterial spore staining
Store temperature: 5℃～30℃
Expiry date: 24 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationMethod limitationPrecautions

Principle

Bacterial spores are the resting state of bacteria. They can resist various physical and chemical factors and endure execrable conditions. Bacterial spores the size, shape and location identification, on the identification of bacteria is of great significance. Spores, which have strong refraction, use strong coloring of Carbolfuchsin,staining under heated conditions, so the dyes not only entered the bacterial, also can enter spores, dye into the bacteria then via the decolorizer was decolorized. Once the spores after staining, decolorizer is difficult to decolor. Then by alkaline methylene blue solution counterstained, spores remain red, and bacteria are stained blue, the spores and bacteria more easily distinguished.

Main components

Reagent composition	Main ingredient
1. Carbolfuchsin solution	Basic fuchsin
2. 95% ethanol	Ethanol
3. Alkaline methylene blue solution	Methylene blue

Sample requirement

Pick colonies and make smear, heat-fixed after natural drying.

Test procedure

1. Add Carbolfuchsin solution on slides. Heat on small flame and keep staining solution steaming for about 5 minutes. After cooling, wash with water.
2. Decolorize with 95% ethanol for 2 minutes and wash with water.
3. Counterstain with Alkaline methylene blue solution for 30 seconds, followed by water wash. Air dry slides and then observe under oil immersion lens.

Result interpretation

Spores	Red
Bacteria	Blue

Method limitation

Only use for morphological staining to observe.

Precautions

1. The age of bacteria for spore staining should be controlled and it is best to use bacteria with spores remained attached.
2. This kit must be used by professionals and the interpretation of results.
3. Should carefully read the instruction manual before using. Can only be used before expiration date, and good personal hygiene protection.
4. After use, disposal of waste should comply with the hospital or the environmental protection department requirements.
5. Tightly cap the reagent bottle immediately after use to avoid evaporation.
6. Production lot number and expiration date on the package.

References

1. The PRC Ministry of Health Medical Administrative Department. National Clinical Laboratory Procedures (M) version 3. Nanjing: Southeast University Press, 2006.

2. Tongming Qing: Clinical Laboratory Pathogenic Microbiology (M). Higher Education Press.

3. Ni Yu-xing: Microbiology and microbiological testing experimental guidance (M). People's Health Publishing House.

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