Lactic Acid Phenol Cotton Blue Stain

Assay-kits

Lactic Acid Phenol Cotton Blue Stain

Product code: ACMBS-56
Specifications: 4x10mL (Box)
Intended use: Used for fungal staining
Store temperature: 5℃～30℃
Expiry date: 24 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationMethod limitationPrecautions

Principle

Fungal hyphae relatively coarse, cell easy shrinkage deformation, and spores are easily dispersed. Lactic acid phenol cotton blue stain solution has producing and staining liquid two effects. Lactic acid phenol cotton bluestain solution added to make the yeast cells, mycelia and sporulation structures can be stained bright blue and observed easily.

Main components

Reagent composition	Main ingredient
Lactic acid phenol cotton blue stain solution	Lactic acid, phenol, cotton blue

Sample requirement

Before samples taken and avoid medication. Different lesion sites, take different specimens.

Test procedure

Take a clean glass slide, dropping a drop of stain solution. Then pick a few cultures or specimens in it, with inoculation needle to spread out. Add cover glass slightly, heating and remove air bubbles with a little pressure then make microscopic examination.

Result interpretation

Fungus is stained blue.

Method limitation

Only use for observed the initial examination of morphological staining.

Precautions

1. This kit must be used by professionals.
2. Should carefully read the instruction manual before using. Can only be used before expiration date, and good personal hygiene protection.
3. After use, disposal of waste should comply with the hospital or the environmental protection department requirements.
4. Tightly cap the reagent bottle immediately after use to avoid evaporation.
5. The production date, production batch number and expiration date on the package.

Reference

1. People's Republic of China national health and Family Planning Commission of medical affairs authority. The clinical laboratory operating procedures M. Fourth edition. Beijing: People's Medical Publishing House, 2014.

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