Mucicarmine Stain

Mucicarmine Stain

Assay-kits

PrincipleMain componentsTest procedureResult interpretationPrecautions

Carmine can bind mordant aluminum to form a complex, which in turn binds to the acid group of mucus and becomes red.

Reagent composition	Main ingredient
1. Hematoxylin solution (Mayer)	Hematoxylin
2. Scotts tap water substitute solution	Magnesium sulfate, sodium bicarbonate
3. Mucicamine solution	Carmine
4. Tartrazine solution	Tartrazine

1. The slices are routinely dewaxed to water and washed once with distilled water.
2. Place in hematoxylin solution (Mayer) for 10 minutes and rinse briefly with running water.
3. Blue in Scotts tap water substitute solution for 1 minute and rinse briefly with running water.
4. Take a clean container, add 1ml Mucicamine solution and 3ml distilled water, mix it into a dilute the red liquid and staining 20～30 minutes.
5. Rinse briefly with running water.
6. Counterstain in Tartrazine solution for 1～3 minutes after blotting up with filter paper.
7. Rinse briefly with running water.
8. Conventional dehydration and transparent, sealed with neutral resin.

1. When hematoxylin is used to stain the nucleus, Ehrlich hematoxylin should be avoided because it can lightly stain the mucus and obstructs mucous coloring.
2. Store Mucicamine solution at 4℃. Put the bottle cap back into the refrigerator after each use, otherwise it will affect its service life.
3. Mucicamine diluent should be prepared prior to use, if placed it at room temperature or refrigerator on the day can also be used, but the effect will be slightly reduced.
4. Tartrazine solution counterfeit dye cannot be excessive, otherwise it will cover the red mucus, but also, can leave out the staining step.
5. This reagent should be used by professionals and interpretation of the results. Read the instruction manual carefully before use, use it within the validity period, and do personal hygiene protection.
6. Dispose of waste according to the requirements of the hospital or environmental protection department after use.
7. The production date, production batch number and expiration date are shown in the outer packaging.

1. Chinese Medical Association. Clinical technical operating specifications-pathology volumes [M]. People's Military Medical Publishing House.

2. Ling Qibo. Practical pathology special staining and histochemistry technology [M]. Guangdong Higher Education Press.

Please fill in the following information and we will process your inquiry as soon as possible.