Mucicarmine Stain

Mucicarmine Stain
Mucicarmine Stain

Assay-kits

Mucicarmine Stain

  • Product code: ACMBS-29
  • Specifications: 4x20mL, 4x100mL (Box)
  • Intended use: Used for the acidic mucous substances staining
  • Store temperature: 2℃~8℃
  • Expiry date: 12 Months

More Details

PrincipleMain componentsTest procedureResult interpretationPrecautions

Principle

Carmine can bind mordant aluminum to form a complex, which in turn binds to the acid group of mucus and becomes red.

Main components

Reagent compositionMain ingredient
1. Hematoxylin solution (Mayer)Hematoxylin
2. Scotts tap water substitute solutionMagnesium sulfate, sodium bicarbonate
3. Mucicamine solutionCarmine
4. Tartrazine solutionTartrazine

Test procedure

  • 1. The slices are routinely dewaxed to water and washed once with distilled water.
  • 2. Place in hematoxylin solution (Mayer) for 10 minutes and rinse briefly with running water.
  • 3. Blue in Scotts tap water substitute solution for 1 minute and rinse briefly with running water.
  • 4. Take a clean container, add 1ml Mucicamine solution and 3ml distilled water, mix it into a dilute the red liquid and staining 20~30 minutes.
  • 5. Rinse briefly with running water.
  • 6. Counterstain in Tartrazine solution for 1~3 minutes after blotting up with filter paper.
  • 7. Rinse briefly with running water.
  • 8. Conventional dehydration and transparent, sealed with neutral resin.

Result interpretation

Acidic mucin substancesRed to rose red
NucleiPurple blue
Other tissue componentsLight yellow

Precautions

  • 1. When hematoxylin is used to stain the nucleus, Ehrlich hematoxylin should be avoided because it can lightly stain the mucus and obstructs mucous coloring.
  • 2. Store Mucicamine solution at 4℃. Put the bottle cap back into the refrigerator after each use, otherwise it will affect its service life.
  • 3. Mucicamine diluent should be prepared prior to use, if placed it at room temperature or refrigerator on the day can also be used, but the effect will be slightly reduced.
  • 4. Tartrazine solution counterfeit dye cannot be excessive, otherwise it will cover the red mucus, but also, can leave out the staining step.
  • 5. This reagent should be used by professionals and interpretation of the results. Read the instruction manual carefully before use, use it within the validity period, and do personal hygiene protection.
  • 6. Dispose of waste according to the requirements of the hospital or environmental protection department after use.
  • 7. The production date, production batch number and expiration date are shown in the outer packaging.

References

1. Chinese Medical Association. Clinical technical operating specifications-pathology volumes [M]. People's Military Medical Publishing House.

2. Ling Qibo. Practical pathology special staining and histochemistry technology [M]. Guangdong Higher Education Press.

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