H-E Consistently High Clarity Stain

Assay-kits

H-E Consistently High Clarity Stain

Product code: ACMBS-03
Specifications: 6x500mL, 6x750mL, 6x1000mL (Box)
Intended use: Mainly used for staining of cell tissue
Store temperature: 10℃～30℃
Expiry date: 15 Months

More Details

PrincipleMain componentsApplicable instrumentSample requirementTest procedureResult interpretationPrecautions

Principle

It is mainly intended to display the common morphological structure for normal components of various tissues and pathological changes, comprehensive observation of the cell tissue. H-E consistently high clarity stain is improved on the basis of traditional H-E staining solution.

It consists of pre-stain solution, hematoxylin solution, differentiation solution (I, II), back to blue solution, eosin solution, composed of five kinds of reagents. Which the pre stain solution on tissue cells were pretreated easy for hematoxylin solution staining. The differentiation solution takes off tissue components hematoxylin stain except the nucleus, it helps to stain eosin later. Back to blue solution let nuclear after acidification become color blue. Hematoxylin and eosin were using tissue cells of different components of physical coloring affinity of different dyes. Therefore, the cells or tissue sections were stained after by H-E consistently high clarity stain, the nucleus is stained with a bright blue purple, the cytoplasm, muscle fiber, collagen fibers showed different levels of red, the red blood cells showed orange red.

Main components

Product type	Reagent composition	Main ingredient
Alcoholic Eosin method	1.Pre-stain solution	Acidifier
	2.Hematoxylin solution	Hematoxylin
	3.Differentiation solution I, Differentiation solution II	Weak acidifier, hydrochloric acid
	4.Back to blue solution	Tris
	5.Eosin solution (Alcoholic)	Eosin
Aqueous Eosin method	1.Pre-stain solution	Acidifier
	2.Hematoxylin solution	Hematoxylin
	3.Differentiation solution I, Differentiation solution II	Weak acidifier, hydrochloric acid
	4.Back to blue solution	Tris
	5.Eosin solution (Aqueous)	Eosin
Differentiation solution II	Differentiation solution II	hydrochloric acid
Hematoxylin solution	Hematoxylin solution	Hematoxylin
Back to blue solution	Back to blue solution	Tris
Eosin solution (Alcoholic)	Eosin solution (Alcoholic)	Eosin
Eosin solution (Aqueous)	Eosin solution (Aqueous)	Eosin

Applicable instrument

This product is suitable for manual staining and auto-stainer staining.

Sample requirement

The thickness of the tissue section is 2~5μm, and must be adequately fixed.

Test procedure

1. Paraffin sections are deparaffinized in xylene (I) (II) (III) for 5 minutes respectively, and then rinsed with absolute ethanol, 95% ethanol, and 80% ethanol for 1 minute each, and rinsed with tap water for 1 minute.
2. Dip section in pre-stain solution for 30 seconds.
3. Stain for 5~8 minutes with Hematoxylin solution. Rinse with water for 2 minutes.
4. Differentiate in differentiation solution I for 20 seconds.
5. Direct differentiate in differentiation solution II for 5~20 seconds. Rinse with water for 2 minutes.
6. Blue with Back to blue solution for 1 minute, and then rinse with water for 1 minute.
7. Eosin solution (Aqueous) staining for 30~60 seconds, washing with water for 10 seconds, if using eosin solution (Alcoholic), the sections should be subjected to 80% ethanol for 1 minute, and then entering into eosin solution(Alcoholic) staining for 10~30 seconds (do not wash in water).
8. Dehydrate in absolute ethanol (I) (II) (III) for 1~2 minutes each.
9. Transparent in xylene (I) (II) (III) for 2 minutes,
10. Sealed with neutral resin, and examine microscopically.

Result interpretation

Nuclei appear indigo blue while cytoplasm, stroma and fibers appear varying shades of red.

Precautions

1. Hematoxylin to avoid low-temperature storage, avoid freezing! It is normal to form a layer of oxidative film on the surface of Hematoxylin and some sediment appears at the bottom of Hematoxylin. With the filter paper can gently scrape oxidative film, the sediment available filter with filter paper, does not affect the subsequent staining effect.
2. When normal use, the kit components consumption rate is the same, convenient for the user to start and stop all the reagents in the kit.
3. The reagent containers shall be cap when not in use, reduce reagent volatilization and excessive contact with air, influence of reagent staining effect.
4. Staining reagent into the container, place up to 2 weeks or up to 2000~2500 pieces staining, should discard these staining solutions.
5. It is hard to stain with Hematoxylin in winter so the staining time should be extended properly.
6. Eosin if staining too deep may be appropriate to extend the time of the first ethanol jar. If the color is lighter, appropriate to extend the Eosin staining time.
7. For kit storage, avoid exposure to extreme high or low temperature and sunlight. In order to avoid to affect the quality and effect.
8. This reagent should be used by professionals and interpretation of the results. Read the instruction manual carefully before use, use it within the validity period, and do personal hygiene protection.
9. Dispose of waste according to the requirements of the hospital or environmental protection department after use.
10. The production date, production batch number and expiration date are shown in the outer packaging.

References

1. Liang Yingjie, Ling Qibo, Zhang Wei. The technology of clinical pathology [M]. People's Medical Publishing House.

2.Gao Mingdu, Huang Yilu. Pathological tissue section technique[M]. Nanshan hall press.

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