Nerve Myelin Stain (Luxol Fast Blue Method)

Nerve Myelin Stain (Luxol Fast Blue Method)
Nerve Myelin Stain (Luxol Fast Blue Method)

Assay-kits

Nerve Myelin Stain (Luxol Fast Blue Method)

  • Product code: ACMBS-36
  • Specifications: 3x20mL, 3x100mL (Box)
  • Intended use: Used for staining of nerve myelin tissue
  • Store temperature: 5℃~30℃
  • Expiry date: 18 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationPrecautions

Principle

Myelin sheath is a tubular sheath that wraps outside the nerve axon. It is segmental, that is, the axon is wrapped segment by segment, and the semi-naked narrow part between every two segments has no myelin sheath, which is called Nodes of Ranvier. Luxol Fast Blue staining solution can show changes such as degeneration, disintegration and loss of myelin sheath under pathological conditions.

Main components

Reagent compositionMain ingredient
1. Luxol fast blue solutionLuxol fast blue
2. Lithium carbonate differentiation solutionLithium carbonate
3. Eosin solutionEosin

Sample requirement

  • The tissue should be fixed quickly.

Test procedure

  • 1. Paraffin sections were deparaffinized procedure until water step.
  • 2. Slightly wash in 95% ethanol.
  • 3. The sections are immersed in Luxol Fast Blue solution and stained in a water bath at 60℃ for 2~3 hours (covered the lid of the dyeing vat) or stained at 37℃ for 18~24 hours.
  • 4. Take out the section, wash the excess staining solution with 75% ethanol and slightly wash it with distilled water.
  • 5. Lithium carbonate differentiation solution is used for 30~60 seconds, until the myelin sheath and background color are different depth (control under the microscope if necessary), and then slightly rinsed with distilled water.
  • 6. Counterstain with Eosin solution for 10~20 seconds, slightly rinse with running water.
  • 7. Routine dehydrated and transparent, and mount with mounting resin.

Result interpretation

  • The nerve myelin sheath is blue-green.

Precautions

  • 1. During the water bath staining process, ensure that the amount of staining solution is enough, the staining temperature is constant, and the staining time is enough, and take care to prevent the tissue from peeling off.
  • 2. Luxol Fast Blue staining solution can be reused 3~4 times, and it must be sealed and stored after each use.
  • 3. The product should be used and the interpretation of the results should be made by professionals.
  • 4. Read the instructions before use, use within the validity period, and keep a personal hygiene protection.
  • 5. After use, the disposal of waste should according to hospital or environmental protection department requires.
  • 6. The production date, production lot number and expiry date are in the packaging.

Reference

1. Wang Detian. Dong Jianqiang. Liang Zhiyong. Practical modern pathology techniques[M]. China Peking Union Medical University Press.

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