Liu's Stain

Liu's Stain

Assay-kits

Product code: ACMBS-11
Specifications: 6x20mL, 4x250mL, 3x100mL (Box)
Intended use: Mainly used for blood cell smears, bone marrow smears, vaginal discharge smears, cell smears staining
Store temperature: 5℃～30℃
Expiry date: 24 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationPrecautions

Principle

Liu's Stain is modified from the Romanowsky Stain technique. Staining of cells involves physical adsorption and chemical affinity which allows stain to penetrate and remain within cells. Because each cell and its components are different in chemical composition, their affinity to acid stain (Eosin) and alkaline stain (Methylene blue) of this kit varies significantly. After staining the smear with the Liu's Stain, different types of cells will appear in different colors. Consequently, one can identify cells based on their stain color, shape and other physical characteristics.

Main components

Reagent composition	Main ingredient
1. Liu A solution	Eosin, Methanol
2. Liu B solution	Methylene blue

Sample requirement

1. Blood cell smear staining: Requires fresh whole blood or EDTA·K2 anticoagulant.
2. Staining of bone marrow smear: After the smear is made, it should be quickly shaken or fanned in the air to prevent the cells from shrinking and deforming, or from hemolysis due to air humidity, and cannot be baked with high temperature or flame.
3. Vaginal secretions (gynecological leucorrhea) smear staining: Fresh specimens leave the body smear, as soon as possible to fix by flame or alcohol to avoid cell deformation.
4. Exfoliated cell smear staining: Take the specimen and smear, the smear can be fixed by natural drying method or wet film fixing liquid fixing method (the specific operation can be based on the specifications of the different specimens and the fixed solution used Operational requirements). Under normal circumstances, if the wet sheet fixation method is used, the specimen soaking time will be slightly longer, and the effect will be better. If wet film fixing liquid are used to fix the specimens, they must be filtered or replaced after use to prevent cross-contamination.

Test procedure

A: Standard stain

1. Add Liu A Solution (about 0.5～0.8 ml) to stain for 30 seconds.
2. Drop Liu B Solution (twice of Liu A Solution) onto Liu A Solution. Blow solutions with rubber pipette bulb to mix thoroughly. Allow to stain for 90～120 seconds.
3. Rinse with water gently, (Reagent cannot be first drained, should be flushed with running water to prevent deposition of sediment on the specimen), dry and examine the finished slide under a microscope.

B: Rapid stain

1. After adding Liu A Solution (about 0.5～0.8 ml), immediately drop Liu B Solution (twice of Liu A Solution) onto Liu A Solution. Blow solutions with rubber pipette bulb to mix thoroughly. Allow to stain for 30～60 seconds.
2. Rinse with water gently, (Reagent cannot be first drained, should be flushed with running water to prevent deposition of sediment on the specimen), dry and examine the finished slide under a microscope.

Result interpretation

1. Blood cell smear, bone marrow smear staining: The red blood cells are light red, and the particles in the white blood cell paste are clear, and they show various colors unique to the cells. The nucleus is purple, and the nuclear chromatin structure is clear.
2. Vaginal secretions (gynecological leucorrhea) staining:

① Trichomonas: After staining with trichomoniasis live trichomoniasis presents a completely different form, under normal circumstances, cannot see flagella. After staining, trichomoniasis is mostly pear-shaped, round, oval or irregular. The pulp is dyed grayish blue or sky blue. The nucleus is small and the nucleus is jujube-like, often biased to one side. Attention should be paid to observation to avoid confusing with epithelial cells.

② Candida (Mould): dark blue, visible spores, pseudohyphae are slender and straight.

③ White blood cells: same as the white blood cells in blood cells smear at morphology and the number of white blood cells suggesting that the level of inflammation, and has a certain index effect for doctor's diagnosis.

④ Ciliates: slender silky, gray-blue.

⑤ Gonococcus: blue, showing a typical kidney-like shape, appearing mostly inside and outside white blood cells and tissue cells. Need to pay attention to female vaginal smear often appear in Staphylococcus, staining results and gonococcus are similar, but also a pair of pairs of double cocci bacteria, so the interpretation of gonococcus need to be very cautious, under normal circumstances, the discovery of gonococcus will be accompanied by a large number The appearance of white blood cells. The interpretation of gonococcal bacteria by this staining solution was only used as a reference for preliminary staining and can' t be the basis for diagnosis.

⑥ Gardnerella: blue, club-shaped. 3. Exfoliated cell staining: The nucleus was stained purple, and the nucleolus stained blue. The cytoplasm varies with the differentiation process, and its basophilic degree also changes, showing varied shades of blue.

Precautions

1. Before use should read the instructions for use, use within the validity period, and keep a personal hygiene protection.
2. After use, the disposal of waste should according to hospital or environmental protection department requires.
3. The production date, production lot number and expiry date are on the packaging.

Reference

1. Gradwohl' s Clinical Laboratory Methods And Diagnosis,8th, ed.

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