COVID-19 RT-qPCR Detection Kit Plus

COVID-19 RT-qPCR Detection Kit Plus
COVID-19 RT-qPCR Detection Kit Plus
COVID-19 RT-qPCR Detection Kit Plus

Catalog numberQP019T-0100

Size100 Reactions

StorageStable for up to 1 year at -20 °C

CategoriesIVD Products

$600 Inquire

Note: COVID-19 / SARS-CoV-2 / RT-qPCR / N1 Gene / TFDA


In view of the joint global efforts of advancing collaborative research in diagnostics, therapeutics, and vaccination in the fight against the COVID-19 (SARS-CoV-2) pandemic, we have specifically developed the LifeDireX COVID-19 RT-qPCR Detection Kit Plus for human respiratory tract specimens.

The COVID-19 RT-qPCR Detection Kit Plus is a multiplex real-time RT-PCR (RT-qPCR) test intended for the qualitative detection of nucleic acid from the SARS-CoV-2 for human respiratory tract specimens (such as nasal swabs, mid-turbinate nasal swabs, nasopharyngeal swabs, oropharyngeal swabs, sputum, lower respiratory tract aspirates, bronchoalveolar lavage, and nasopharyngeal wash/aspirate or nasal aspirate) from individuals suspected of COVID-19 by their healthcare provider. Results are for the identification of SARS-CoV-2 RNA.

The kit is characterized by:

  • High specificity for the N target markers as recommended by WHO and US CDC.
  • Data obtained in less than 2 hours.
  • Compatible with standard RT-qPCR machines.

Kit Contents

ComponentDescriptionAmount Supplied (per 100 rnxs)
2X RT-qPCR MasterMixMultiplex assay primers/probes for N1 and RP (human) genes with HotStart DNA polymerase, dNTPs, and buffer1000 μl x 1
RScript Enzyme MixRScript reverse transcriptase with RIBOAssure RNase inhibitor20 μl x 1
Positive ControlPseudo-virus DNA containing N1 gene50 μl x 1
Negative Extraction ControlCancer cell line with RP gene1 ml x 1
Nuclease-Free WaterDEPC-treated water1 ml x 1
  • Gene Expression (mRNA) Analysis
  • Copy Number Analysis
  • SNP Genotype Analysis
Storage and Handling Requirements
  • COVID-19 RT-qPCR Detection Kit Plus is shipped with dry ice and gel packs.
  • All components of the kit arrive in solution.
  • All components of the kit must be stored at -20 °C upon arrival.
  • Do not use kit components after expiration date printed on the box label.
  • If there is damage to the packaging inside, outside or kit contents have been tempered with, or storage condition failed to meet above -20 °C, do not use.
  • Dispose of unused reagents and waste in accordance with country, federal, state, and local regulations.
  • Repeated freezing and thawing may lead to inaccurate results.
  • The kit is stable for up to 1 year from date of release.

Note: Inaccurate results may be obtained if the kit is not handled according to the instructions provided.

Interpretation of Results
++/-PositiveReport results to health authority.
-+NegativeSARS-CoV-2 not detected. Report results to helath authority.
--Invalid ResultRepeat from extraction step. If the repeated result remains invalid, recommend collection of a new specimen(s) from the patient.

A study was performed to assess the performance of COVID-19 RT-qPCR Detection Kit Plus. A testing of 20 replicates at the tentative Limit of Detection (LoD) concentration was carried out with 50 copies/reaction at 95% detection rate. Samples were spiked SARS-CoV-2 RNA in pooled negative clinical nasopharyngeal swab matrix, then extracted with QIAamp Viral RNA Mini Kit and tested on QuantStudio™ 5 Real-Time PCR System. The synthetic SARS-CoV-2 RNA was obtained from Twist Biosciences (Assay Ready Control 14,EPI_ISL_710528).

Limit of Detection (LoD)

Verification code
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