Blu12 (BLUeye) Prestained Protein Ladder

Blu12 (BLUeye) Prestained Protein Ladder
Blu12 (BLUeye) Prestained Protein Ladder
Blu12 (BLUeye) Prestained Protein Ladder

Catalog numberPMB12-0500 / PM007-0500

Size500 μl

Range11 to 245 kDa

UseAs molecular weight standards for SDS-PAGE electrophoresis

Sample25 μl ($7.2)

$144 Inquire

Note: Protein Marker / Protein Standard / SDS-PAGE / Western Blot


The Blu12 (BLUeye) Prestained Protein Ladder is a combination of 12 pre-stained proteins with molecular weights from 11 to 245 kDa. The 12 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The Blu12 (BLUeye) Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.


  • Appearance: Liquid
  • Color: Blue
  • Odor: Odorless
  • Weak pH-value at 25 °C: ~7.5
  • Boiling point: ~ 100 °C

Reaction Setup

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Guide for Molecular Weight Estimation (kDa)

Refer to the Blu12 (BLUeye) Prestained Protein Ladder patterns in various electrophoresis conditions:

Blu12 (BLUeye) Prestained Protein Ladder

Important notes: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Storage Buffer

Approximately 0.1~0.5 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25 °C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

Storage Conditions
  • Stable for up to 2 weeks at 25 °C.
  • Stable for up to 3 months at 4 °C.
  • Stable for up to 24 months at -20 °C.
Required Materials but Not Provided
  • Vertical Electrophoresis system
  • Power supplies
  • Vortex or equivalent
  • Microcentrifuge
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