Blu10 Plus (BLUltra) Prestained Protein Ladder

Blu10 Plus (BLUltra) Prestained Protein Ladder
Blu10 Plus (BLUltra) Prestained Protein Ladder
Blu10 Plus (BLUltra) Prestained Protein Ladder

Catalog numberPMB01-0500 / PM001-0500

Size500 μl

Range6.5 to 270 kDa

UseAs molecular weight standards for SDS-PAGE electrophoresis

Sample25 μl ($7.2)

$132 Inquire

Note: Protein Marker / Protein Standard / SDS-PAGE / Western Blot / Protein Electrophoresis

Description

The Blu10 Plus (BLUltra) Prestained Protein Ladder is a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. The Blu10 Plus (BLUltra) Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Properties

  • Appearance: Liquid
  • Color: Dark blue
  • Odor: Odorless
  • pH-value: Not determined
  • Boiling point: Undetermined

Reaction Setup

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Guide for Molecular Weight Estimation (kDa)

Migration patterns of Blu10 Plus (BLUltra) Prestained Protein Ladder in different electrophoresis conditions are listed below:

Blu10 Plus (BLUltra) Prestained Protein Ladder

Important notes: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Storage Buffer

0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

Storage Conditions
  • Stable for up to 2 weeks at 25 °C.
  • Stable for up to 3 months at 4 °C.
  • Stable for up to 24 months at -20 °C.
Required Materials but Not Provided
  • Vertical Electrophoresis system
  • Power supplies
  • Vortex or equivalent
  • Microcentrifuge
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