UltraScence Femto Western Substrate

UltraScence Femto Western Substrate
UltraScence Femto Western Substrate
UltraScence Femto Western Substrate

Catalog numberCCH365-B100ML

Size100 ml (50 ml×2); 600 ml

Substrate TypeHRP (Horseradish Peroxidase) Substrate

Sample4 ml (2 ml x 2) ($7.2)

$184.8 (100 ml)$924 (600 ml)Inquire

Note: Enhanced Chemiluminescent / HRP (Horseradish Peroxidase) / ECL substrates / Femtogram / Luminol / Peroxide Buffer / Western Blotting


The UltraScence Femto Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The mid femtogram to low femtogram detection of antigen is enabled by UltraScence Femto Western Substrate's excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.

UltraScence Femto Western Substrate

  • No optimization required. Switching to the UltraScence Pico Plus Western ECL Substrate from other brands, such as Pierce ECL and GE Healthcare, does not require optimization or protocol changes.
  • High degree of sensitivity and enhanced chemiluminescence duration. UltraScence Pico Plus Western ECL Substrate enables an accurate low picogram to high femtogram detection of protein on the same immunoblot after a single exposure.
  • Optimized for use with PVDF and nitrocellulose membranes.
  • Compatible with Western Blotting Markers.
  • Optimized for film- and CCD-based imaging.
  • Patent pending product.
Storage and Shipping
  • Stable for up to 24 months at 4 °C, do not freeze it.
  • Ship at 4 °C, beware of shipping in any condition beneath 0 °C.
Reaction Setup

1. Keep the membrane moist in the wash buffer while preparing the substrate mixture. Please ensure the membrane does not dry out during the subsequent steps.
2. Mix Luminol solution and Peroxide Solution in a 1:1 ratio, and thoroughly agitate the chemiluminescent substrate solution well for preparing the 0.1 ml of solution / cm2 of membrane.
- For a mini-sized membrane (7 x 8.5 cm), 4 ml of solution is sufficient.
- For a midi-sized membrane (8.5 x 13.5 cm), 10 ml of solution is sufficient.
3. Place the membrane with the protein side up on a clear and level surface or in a clean container.
4. Remove the membrane from the chemiluminescent substrate solution and drain off excessive solution.
5. Place the membrane in a plastic sheet protector or in plastic wrap to prevent the membrane from drying.
6. Image the membrane with a digital imager or by exposing to the X-ray film.

UltraScence Femto Western Substrate

Required Materials but Not Provided
  • A compatible Chemiluminescence or X-ray Imaging Systems.
  • A plastic sheet protector or plastic wrap to prevent the membrane from drying.
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