UNveil Unstained Protein Ladder

Catalog numberPMU12-0500

Size500 μl

Range10 to 200 kDa

UseAs molecular weight standards for SDS-PAGE electrophoresis

Sample25 μl ($7.2)

$105.6 Inquire

Note: Unstained / Protein Marker / Protein Standard / SDS-PAGE / Western Blot / Staining Reagent / Coomassie Blue

Description

The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.

Properties

Appearance: Liquid
Color: Blue
Odor: Odorless
pH-value: 7.5
Boiling point: Undetermined

Reaction Setup

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Guide for Molecular Weight Estimation (kDa)

Migration patterns of Blu10 (BlueRAY) Prestained Protein Ladder in different electrophoresis conditions are listed below:

UNveil Unstained Protein Ladder

Important notes: The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.

Storage Buﬀer

20 mM Tris-phosphate(pH 7.5 at 25 °C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.

Quality Control

Under suggested conditions, Unveil Unstained Protein Ladder resolves 12 major bands in 4-12%Bis- Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane.

Storage Conditions

Stable for up to 2 weeks at 25 °C.
Stable for up to 3 months at 4 °C.
Stable for up to 24 months at -20 °C.

Required Materials but Not Provided

Vertical Electrophoresis system
Power supplies
Vortex or equivalent
Microcentrifuge