Mbead Virus Genomic Nucleic Acid Kit

MBead Virus Genomic Nucleic Acid Kit was designed specifically for genomic DNA/RNA isolation from Virus samples. Its special buffer system will efficiently lyse cell and degrade protein, allowing for DNA/RNA to be easily bound by the surface of the magnetic beads. The other non-specific binding particles are removed with a wash buffer, and the genomic DNA/RNA is released from the beads by addition of a low ionic strength buffer and heat. Genomic DNA/RNA can be purified manually within 15 minutes (using most magnetic separators) or the kit can be easily adapted to satisfy most automated Nucleic Acid purification systems.

• Sample: Up to 300 μl of the virus sample
• Operation time: Within 10-15 minutes
• Applications: Restriction Enzyme Digestion, Southern Blotting, PCR and qPCR assays
• Storage: Room temperature

Kit Contents

Step 1 Lysis

1. Transfer up to 300 µl of the virus sample into a 1.5 ml microcentrifuge tube and add 300 µl of the Lysis Buffer.
2. Mix well and incubate at 65°C for 5 minutes. During this time, pre-heat the Release Buffer to 65°C for the Step 4.
3. Add 300 µl of the absolute EtOH to the lysate and mix well.

Step 2 DNA Binding

1. Add 20 µl of the Magnetic Beads. Mix well by gently shaking for 3 minutes.
2. Place the tube in a magnetic separator for 30 seconds.
3. Remove the solution (If the mixture becomes viscous, increase magnetic bead separtion time).

Step 3 Wash

1. Add 800 µl of the Wash Buffer and mix well (Following the wash, the mixture will no longer be viscous).
2. Place the tube in a magnetic separator for 30 seconds. Remove the solution.

Step 4 Release

1. Add 200 µl of the Release Buffer (pre-heated to 65°C) and mix well.
2. Incubate for 3 minutes at 65°C (During the incubation, shake the tube vigorously every minute).
3. Place the tube in a magnetic separator for 1 minute.
4. Carefully transfer ONLY the clean portion of the solution to a clean tube.

Refer to the table below to troubleshoot problems that you may encounter when purifying the genomic DNA with the kit.

• During the operation, always wear a lab coat, disposable gloves, and protective equipment.
• Research Use Only. Not intended for any animal or human therapeutic or diagnostic uses.