Mbead Virus Genomic Nucleic Acid Kit

Mbead Virus Genomic Nucleic Acid Kit
Mbead Virus Genomic Nucleic Acid Kit
Mbead Virus Genomic Nucleic Acid Kit

Catalog numberPDM04-0100

CategoriesMbeads Based

Size100 reactions

Sample4 rxns ($21.6)

$369.6 Inquire

Note: Purification Kits / Magnetic Beads / Extraction Kit / Isolation Kit / Virus / COVID-19 / SARS-CoV-2


MBead Virus Genomic Nucleic Acid Kit was designed specifically for genomic DNA/RNA isolation from Virus samples. Its special buffer system will efficiently lyse cell and degrade protein, allowing for DNA/RNA to be easily bound by the surface of the magnetic beads. The other non-specific binding particles are removed with a wash buffer, and the genomic DNA/RNA is released from the beads by addition of a low ionic strength buffer and heat. Genomic DNA/RNA can be purified manually within 15 minutes (using most magnetic separators) or the kit can be easily adapted to satisfy most automated Nucleic Acid purification systems.

  • Sample: Up to 300 μl of the virus sample
  • Operation time: Within 10-15 minutes
  • Applications: Restriction Enzyme Digestion, Southern Blotting, PCR and qPCR assays
  • Storage: Room temperature

Kit Contents

Magnetic Bead2 ml
Lysis Buffer30 ml
Wash Buffer80 ml
Release Buffer20 ml

Step 1 Lysis

1. Transfer up to 300 µl of the virus sample into a 1.5 ml microcentrifuge tube and add 300 µl of the Lysis Buffer.
2. Mix well and incubate at 65°C for 5 minutes. During this time, pre-heat the Release Buffer to 65°C for the Step 4.
3. Add 300 µl of the absolute EtOH to the lysate and mix well.

Step 2 DNA Binding

1. Add 20 µl of the Magnetic Beads. Mix well by gently shaking for 3 minutes.
2. Place the tube in a magnetic separator for 30 seconds.
3. Remove the solution (If the mixture becomes viscous, increase magnetic bead separtion time).

Step 3 Wash

1. Add 800 µl of the Wash Buffer and mix well (Following the wash, the mixture will no longer be viscous).
2. Place the tube in a magnetic separator for 30 seconds. Remove the solution.

Step 4 Release

1. Add 200 µl of the Release Buffer (pre-heated to 65°C) and mix well.
2. Incubate for 3 minutes at 65°C (During the incubation, shake the tube vigorously every minute).
3. Place the tube in a magnetic separator for 1 minute.
4. Carefully transfer ONLY the clean portion of the solution to a clean tube.

Mbead Virus Genomic Nucleic Acid Kit


Refer to the table below to troubleshoot problems that you may encounter when purifying the genomic DNA with the kit.

DNA is sheared or degradedLysate mixed too vigorouslyUse the appropriate pipette tip set. For the required volume, lower under the reading line of the solution to mix the sample. Pipet up and down gently to mix.
DNase contaminationMaintain a sterile environment while working (e.g. wear gloves and use DNase-free reagents).
Incomplete removal of the RNaseRNase A treatment
RNA containmentIncomplete lysis and homogenizationReduce the amount of the starting material.
Low yields of gDNAIncorrect handing of Magnetic BeadsVortex the tube containing the magnetic beads to fully resuspend the beads before adding them to your sample.
Incorrect elution conditionsDuring the Elution Step, incubate at 65 ℃for 3 minutes, and vortex every minute.
The quality of the starting material may not be optimalUse the fresh sample and process immediately after collection, or freeze the sample at -80℃or in liquid nitrogen.
High background on UV measurementResidual beads releasedRepeat magnetic separation and transfer the eluate to a clean tube
  • During the operation, always wear a lab coat, disposable gloves, and protective equipment.
  • Research Use Only. Not intended for any animal or human therapeutic or diagnostic uses.
Verification code
Contact Us

Send Us a Request

What is your specific need? We will do everything we can to meet your expectations.
Online Inquiry
Privacy Policy | Cookie Policy | Copyright © 2024 Alfa Chemistry. All rights reserved.