Genomic DNA Isolation Kit (Tissue)

Catalog numberPDC11-0100

CategoriesColumn Based

Size100 Reactions

Sample4 rxns ($19.2)

$316.8 Inquire

Note: PureDireX / Purification Kits / Extraction Kit / Tissue

Description

The Genomic DNA Isolation Kit (Tissue) is designed specifically for genomic DNA isolation from animal tissue samples. This unique buffer system ensures total DNA with high yield and good quality from samples. The spin column system is designed to purify and concentrate DNA products which have been previously isolated using buffers. The entire procedure can be completed in 1 hour without phenol/chloroform extraction. Purified DNA is suitable for use in PCR or other enzymatic reactions.

Sample: 30 mg of fresh animal tissue.
Format: spin column.
Column capacity: up to 50 µg.
Operation time: within 60 minutes.

Kit Contents

Contents	PDC11-0100
Buffer TL	35 ml
Buffer TP	12 ml
Buffer W1	45 ml
Buffer W2 (Add ethanol)	15 ml (60 ml)
Buffer EL	10 ml
Column TC	100 pcs
Collection Tubes	100 pcs

Features

Delivering high-quality genomic DNA with the fast procedure.
Ready-to-use gnomic DNA for high performance in any downstream application.
Highly purified and high yield genomic DNA can be extracted from various samples.
Optimized lysis buffer for the efficient lysis.
Designed to rapidly purify high-quality DNA using spin column format.

Application

Gene cloning
PCR

Southern blotting
SNP genotyping

Protocol

Fresh Tissue

Genomic DNA Isolation Kit (Tissue)

Troubleshooting

Refer to the table below to troubleshoot problems that you may encounter when purifying the genomic DNA with the kit.

Problem	Cause	Solution
Low yield of DNA	Incomplete lysed sample	Use the appropriate method for the lysate preparation based on the amount of the starting materials.
Low yield of DNA	Incomplete lysed sample	Increase the digestion time.
DNA degrade	Sample not fresh	Avoid repeated freeze / thaw cycles of the sample.
	Sample not fresh	Use a new sample for the DNA isolation. Perform the extraction of the fresh material when possible.
	Inappropriate sample storage conditions	Store culture cell / fungus at -20°C until use. The whole blood can be stored at 4°C for no longer than 1-2 days.
	DNase contaminant	Use the fresh TAE or TBE electrophoresis buffer.
	DNase contaminant	Maintain a sterile work environment to avoid contamination from DNases.
Presence of RNA	RNA contamination	Perform RNase A digestion step during Step Lysis.
Inhibition of downstream enzymatic reactions	Presence of ethanol in purified DNA	Discard the ethanol of the Buffer W2 flow through from the collection tube. Place the spin cartridge into the collection tube and centrifuge the spin cartridge at maximum speed for 2–3 minutes to completely dry the cartridge.

Caution

During operation, always wear a lab coat, disposable gloves, and protective equipment.
Check buffers before use for salt precipitation. Redissolve any precipitate by warming to 37°C.
Add 60 ml of the ethanol (96–100%) to Buffer W2, and shake before use.
Buffers W1 contain irritants. Wear gloves when handling these buffers.
Research Use Only. Not intended for any animal or human therapeutic or diagnostic uses.

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