Genomic DNA Isolation Kit (Tissue)

Genomic DNA Isolation Kit (Tissue)
Genomic DNA Isolation Kit (Tissue)
Genomic DNA Isolation Kit (Tissue)

Catalog numberPDC11-0100

CategoriesColumn Based

Size100 Reactions

Sample4 rxns ($19.2)

$316.8 Inquire

Note: PureDireX / Purification Kits / Extraction Kit / Tissue


The Genomic DNA Isolation Kit (Tissue) is designed specifically for genomic DNA isolation from animal tissue samples. This unique buffer system ensures total DNA with high yield and good quality from samples. The spin column system is designed to purify and concentrate DNA products which have been previously isolated using buffers. The entire procedure can be completed in 1 hour without phenol/chloroform extraction. Purified DNA is suitable for use in PCR or other enzymatic reactions.

  • Sample: 30 mg of fresh animal tissue.
  • Format: spin column.
  • Column capacity: up to 50 µg.
  • Operation time: within 60 minutes.

Kit Contents

Buffer TL35 ml
Buffer TP12 ml
Buffer W145 ml
Buffer W2 (Add ethanol)15 ml (60 ml)
Buffer EL10 ml
Column TC100 pcs
Collection Tubes100 pcs
  • Delivering high-quality genomic DNA with the fast procedure.
  • Ready-to-use gnomic DNA for high performance in any downstream application.
  • Highly purified and high yield genomic DNA can be extracted from various samples.
  • Optimized lysis buffer for the efficient lysis.
  • Designed to rapidly purify high-quality DNA using spin column format.
  • Gene cloning
  • PCR
  • Southern blotting
  • SNP genotyping
  • Fresh Tissue

Genomic DNA Isolation Kit (Tissue)


Refer to the table below to troubleshoot problems that you may encounter when purifying the genomic DNA with the kit.

Low yield of DNAIncomplete lysed sampleUse the appropriate method for the lysate preparation based on the amount of the starting materials.
Increase the digestion time.
DNA degradeSample not freshAvoid repeated freeze / thaw cycles of the sample.
Use a new sample for the DNA isolation. Perform the extraction of the fresh material when possible.
Inappropriate sample storage conditionsStore culture cell / fungus at -20°C until use. The whole blood can be stored at 4°C for no longer than 1-2 days.
DNase contaminantUse the fresh TAE or TBE electrophoresis buffer.
Maintain a sterile work environment to avoid contamination from DNases.
Presence of RNARNA contaminationPerform RNase A digestion step during Step Lysis.
Inhibition of downstream enzymatic reactionsPresence of ethanol in purified DNADiscard the ethanol of the Buffer W2 flow through from the collection tube. Place the spin cartridge into the collection tube and centrifuge the spin cartridge at maximum speed for 2–3 minutes to completely dry the cartridge.
  • During operation, always wear a lab coat, disposable gloves, and protective equipment.
  • Check buffers before use for salt precipitation. Redissolve any precipitate by warming to 37°C.
  • Add 60 ml of the ethanol (96–100%) to Buffer W2, and shake before use.
  • Buffers W1 contain irritants. Wear gloves when handling these buffers.
  • Research Use Only. Not intended for any animal or human therapeutic or diagnostic uses.
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