nanoTaq Hot-Start DNA Polymerase

nanoTaq Hot-Start DNA Polymerase
nanoTaq Hot-Start DNA Polymerase
nanoTaq Hot-Start DNA Polymerase

Catalog numberDP001-0100

Size100 μl

Concentration5 units/μl

StorageStable for up to 1 year at -20 °C


Note: Hot-Start Taq / Universal Annealing Protocol / Thermostability / COVID-19 (SARS-CoV-2) / qPCR / real-time PCR / nano technology / Amplification


nanoTaq DNA Polymerase was designed as one of enhanced hot start enzyme DNA Polymerases which provide the convenience and reliability toward your research destination. nanoTaq was engineered with nano technology complex, which is an innovative creation, different from the traditional methods of hot start enzymes made. The proven features of nanoTaq covers reactions at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases, reducing nonspecific primer annealing, improving product yield and using for PCR products up to 5 kb.

  • High tolerance aganist various reaction inhibitors
  • Reduce nonspecific primer annealing
  • Plays in efficient PCR amplification of GC-rich sequences
  • Using the same protocol and cycling conditions as conventional Taq DNA polymerases
Reaction Setup
  • For each 20 μl reaction, assemble the following in a 0.2 ml PCR tube on ice just prior to use:
VolumeFinal Conc.
DNA template- μl3 ng
Forward primer, 5-10 μM- μl0.1-0.5 μM
Reverse primer, 5-10 μM- μl0.1-0.5 μM
dNTP Mix (10 mM each dATP, dCTP, dGTP, dTTP)- μl200 µM
10X PCR buffer2 μl-
NanoTaq Hot-Start DNA Polymerase-1 μl-
PCR Grade WaterAdd to 20 μl-
Total volume20 μl
  • Mix gently. If necessary, centrifuge briefly and cap tubes.
  • Place them into Thermocycler and process for 30-35 cycles as follows:
Initial Denaturation3 min at 95 °C
Denaturation30 sec
Annealing30 sec at the proper annealing temperature
Extension1 min at 72 °C
Final extension5 min at 72 °C
nanoTaq Hot-Start DNA Polymerase

Important notes: Optimal conditions for amplification will vary depending on the primers and thermal cycler used. It may be necessary to optimize the system for individual primers, template, and thermal cycler.


Purified high quality DNA is needed for a success PCR reaction.

Storage Buffer

The enzyme is supplied in a storage buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol, and 1% Triton X-100.

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid- insoluble form in 30 min at 74 °C in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino- propane-sulfonic acid, sodium salt), pH 9.3 at 25 °C, 50 mM KCl, 2 mM MgCl2, 1 mM β-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 μM [α-32P] dCTP, and activated salmon sperm DNA.

Required Materials but Not Provided
  • A compatible PCR instrument
  • Vortex or equivalent
  • Microcentrifuge
  • Plates and seals for your instruments
Verification code
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