^nanoTaq Hot-Start DNA Polymerase

^nanoTaq DNA Polymerase was designed as one of enhanced hot start enzyme DNA Polymerases which provide the convenience and reliability toward your research destination. nanoTaq was engineered with nano technology complex, which is an innovative creation, different from the traditional methods of hot start enzymes made. The proven features of nanoTaq covers reactions at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases, reducing nonspecific primer annealing, improving product yield and using for PCR products up to 5 kb.

• High tolerance aganist various reaction inhibitors
• Reduce nonspecific primer annealing
• Plays in efficient PCR amplification of GC-rich sequences
• Using the same protocol and cycling conditions as conventional Taq DNA polymerases

• For each 20 μl reaction, assemble the following in a 0.2 ml PCR tube on ice just prior to use:

• Mix gently. If necessary, centrifuge briefly and cap tubes.
• Place them into Thermocycler and process for 30-35 cycles as follows:

Initial Denaturation	3 min at 95 °C
Denaturation	30 sec
Annealing	30 sec at the proper annealing temperature
Extension	1 min at 72 °C
Final extension	5 min at 72 °C

Template

Purified high quality DNA is needed for a success PCR reaction.

Storage Buffer

The enzyme is supplied in a storage buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol, and 1% Triton X-100.

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid- insoluble form in 30 min at 74 °C in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino- propane-sulfonic acid, sodium salt), pH 9.3 at 25 °C, 50 mM KCl, 2 mM MgCl₂, 1 mM β-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 μM [α-32P] dCTP, and activated salmon sperm DNA.

• A compatible PCR instrument
• Vortex or equivalent
• Microcentrifuge
• Plates and seals for your instruments