OmniMARK (BH OneMARK) 100 RTU

OmniMARK (BH OneMARK) 100 with the Novel Green was designed to show virtually uniform spacing over a wide fragment range. The ladder is supplied in a ready-to-use format containing the fluorescent DNA stain and tracking dyes. High quantum yield and excellent stability make the fluorescence dye the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes, ethidium bromide or SYBR^® Green I.

The OmniMARK (BH OneMARK) 100 with the fluorescent stain was optimized for direct loading onto unstained agarose gels. The ladders provide highest level of convenience during the routine handling and avoid commonly used gel staining procedures with ethidium bromide or SYBR^® Green I.

The OmniMARK (BH OneMARK) 100 includes fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.54 μg per loading) for approximating the mass of DNA in comparably intense samples of similar size.

Source

PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Reaction Setup

Direct loading 6 μl / well onto agarose gel, it contains orange G & xylene cyanol FF as tracking dyes, and Novel Green fluorescence dye for producing an instant visualization of DNA bands upon illuminating agarose gels on the Blue Light or UV Transilluminator. Please note, the ladder is light sensitive and should be stored and protected from light.

Application

No-post-staining procession.
Direct loading onto your agarose gel for analysis.

• Stable for up to 6 months at 25 °C.
• Stable for up to 12 months at 4 °C.
• Stable for up to 24 months at -20 °C

• Horizontal Electrophoresis system
• Power supplies
• Vortex or equivalent
• Microcentrifuge