Oil Red O Stain

Precautions

• 1. After the frozen section is prepared, it can be stained without fixing again, it can also be fixed in 10% formaldehyde solution for 1 to 2 minutes, and the fixation time should not be too long to avoid lipid dissolution.
• 2. Since fats are readily soluble in organic solvents, it is generally not shown that fats can be treated like paraffin sections, but rather displayed by frozen section staining.
• 3. The frozen tissue used for lipid staining should not be too thin. Otherwise, the lipid content may be lost.
• 4. After the Oil red O working solution is prepared, it should be dyed after standing for 10 to 20 minutes. If the standing time is too long, it may cause light dyeing. Precipitated particles will precipitate and sink to the bottom of the container, so try to use the upper working solution, to avoid precipitation of the particles.
• 5. The counterstaining time in hematoxylin solution (Mayer) should not be too long.
• 6. The tissue staining will not last for too long, so observe and take pictures as soon as possible.
• 7. This reagent should be used by professionals and interpretation of the results. Read the instruction manual carefully before use, use it within the validity period, and do personal hygiene protection.
• 8. Dispose of waste according to the requirements of the hospital or environmental protection department after use.
• 9. The production date, production batch number and expiration date are shown in the outer packaging.