Urinary Sediment Stain (S-M Method) & (S Method)

Urinary Sediment Stain (S-M Method) and (S Method)

Assay-kits

Urinary Sediment Stain (S-M Method) & (S Method)

Product code: ACMBS-61
Specifications: 3x20mL (Box)
Intended use: This kit is for staining urine sediment smear
Store temperature: 5℃～30℃
Expiry date: 18 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationPrecautions

Principle

Directly add stain into urinary sediment, and the components in urinary sediment can be easily identified based on their distinct shape and structure. Compared with the traditional S-M stain, this Urinary sediment stain offers the benefits of shorter staining time and longer storage stability at room temperature.

Main components

Product type	Reagent composition	Main ingredient
S-M method	Urinary Sediment Stain (S-M method)	Crystal Violet, Safranin O
S method	Urinary Sediment Stain (S method)	Pyronin B, Alcian blue

Sample requirement

Urinary specimen must be fresh and morning urine is the best. Specimen should be tested within 1 hour after collection. Otherwise, add formaldehyde to the specimen and store at 4℃ until testing.

Test procedure

1. Place 10 ml fresh urine into a conical centrifuge tube. Centrifuge at 2,000 rpm for 5 minutes.
2. Decant the supernatant and retain 0.2 ml of urinary sediment at the bottom of the tube.
3. Add one drop of stain into the urinary sediment tube and slightly mix gently, and wait for 1 minute.
4. Transfer a drop of the stained sediment to a slide, add a cover slip and observe microscopically. Examine the distributions of cells and casts under low power lens. Enumerate cells with at least 10 fields under high power lens and report the average value. Enumerate casts with 20 fields at least under low power lens and report distributions profile according to the fields under high power magnification.

Result interpretation

S-M method:

① Neutrophilic leukocytes are stained purple, nuclei are stained red-purple.
② Vaginal squamous epithelial cells are stained pale purple, nuclei are stained dark purple.
③ Bladder epithelial cells are colorless or pale blue.
④ Hyaline casts are stained pink or light purple.
⑤ Granules are stained red and purple, Granular casts are stained blue.
⑥ Living and active bacteria are stained pink, dead bacteria are stained dark purple.
⑦ Cells of yeasts are stained dark purple or unstained.
⑧ Erythrocytes are stained light red-purple.

S method:

① Erythrocytes are stained red or colorless.
② Leukocytes are stained dark blue or pale blue or colorless.
③ Squamous epithelial cells are stained pale pink or red-purple.
④ Transitional epithelial cells, renal tubular epithelial cells are stained purple.
⑤ Cell casts are stained light or dark blue.
⑥ Granular casts are stained pink or purple.

Precautions

1. If urine is alkalescent, adjust to slight acidic with 1% acetic acid to clear phosphate away. Should take care not to add too much acid, or else erythrocytes and casts will be dissolved.
2. Prior to urine collection, it is very important to ensure that patients follow proper fasting procedure (for example, no drinking water during specimen collection), or else urine will be diluted and results not reliable. For adult women, their pudendum should be cleaned before collection. It is preferred to collect the mid-stream of urine sample in order to avoid introducing any pudendum and castoff cells from the vagina.
3. The staining time should be suitable. If stain for too long, the lightly stained cells can cause the transition of light staining cells to concentrated staining.
4. For bilirubin urine, its tangible components can be stained yellow that masks their true color. In addition, the pigment granules of stain may also be mistaken as components of urinary sediment. Hence, it is important to differentiate all components carefully to avoid any misidentification.
5. This kit must be used by professionals and the interpretation of results.
6. Should carefully read the instruction manual before using. Can only be used before expiration date, and keep a good personal hygiene protection.
7. After use, disposal of waste should comply with the hospital or the environmental protection department requirements.
8. Production lot number and expiration date are on the package.

References

1. The PRC Ministry of Health Medical Administrative Department. National Clinical Laboratory Procedures (M) version 3. Nanjing: Southeast University Press, 2006.

2. Gradwohl's Clinical Laboratory Methods And Diagnosis, 8th, ed.

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