Papanicolaou Stain

Papanicolaou Stain
Papanicolaou Stain


Papanicolaou Stain

  • Product code: ACMBS-09
  • Specifications: 3x250mL, 1000mL (Box)
  • Intended use: Intended for histocytological staining of exfoliated cells
  • Store temperature: 10℃~30℃
  • Expiry date: 15 Months

More Details

PrincipleMain componentsTest procedureResult interpretationPrecautions


The nucleus in the cell is composed of acidic substances, it has a strong affinity with the basic dye, and the cytoplasm is the opposite, it contains alkaline substances and has a strong affinity with the acid dye. Papanicolaou staining solution using this feature on the cell coloring, and the cellular structure can be clearly stained. Cytoplasm is translucent bright; a variety of particles and the nucleus chromatin are clear. So, it's easier to find abnormal cells. Papanicolaou staining can reflect the morphological changes of cell after inflammatory stimulation and cancer. It has a great significance of detection and diagnosis of some lesions and tumors in the early.

Main components

Product typeReagent compositionMain ingredient
EA36Hematoxylin Solution (Harris)Hematoxylin
Orange G SolutionOrange G
EA36 SolutionBright green, Eosin
EA50Hematoxylin Solution (Harris)Hematoxylin
Orange G SolutionOrange G
EA50 SolutionBright green, Eosin
Orange G SolutionOrange G SolutionOrange G
EA36 SolutionEA36 SolutionBright green, Eosin
EA50 SolutionEA50 SolutionBright green, Eosin
OG/EA SolutionOG/EA SolutionOrange G, Bright green, Eosin

Test procedure

Test procedureTime EA36; EA50 OG/EA
1Fix smears in 95% ethanol15 minutes15 minutes
2Dip smears in 75% or 80% ethanol30 seconds30 seconds
3Dip smears in 50% ethanol30 seconds30 seconds
4Rinse with water gently30 seconds30 seconds
5Hematoxylin stain (Harris)3~5 minutes3~5 minutes
6Rinse with water gently1~2 minutes1~2 minutes
7Differentiate in 0.5%~1% hydrochloric acid ethanolSeveral secondsSeveral seconds
8Blue with running water (or with dilute lithium carbonate solution)5~10 minutes (or 30 seconds)5~10 minutes (or 30 seconds)
9Rinse with water gently1~2 minutes1~2 minutes
10Dip smears in 50% ethanol30 seconds30 seconds
11Dip smears in 75% or 80% ethanol30 seconds30 seconds
12Dip smears in 95% ethanol2 minutes2 minutes
13Orange G Solution1~2 minutes——
14Dip smears in 95% ethanol I、II10~20 seconds each.——
15EA36 Solution; EA50 Solution; OG/EA Solution5~8minutes5~8minutes
16Dip smears in 95% ethanol I、II10~20 seconds each.10~20 seconds each.
17Dip smears in 100% ethanol I、II30~60 seconds each.30~60 seconds each.
18Conventional dehydration and transparent, sealed with neutral resin

Result interpretation

EpitheliaNuclear is indigo and nucleolus is red, keratinized cells in cytoplasm appear pink, full-keratinized cells appear orange, cells prior to keratinization are sky-blue or light green.
ErythrocyteVermeil or salmon pink.
LeukocyteCytoplasm is sky-blue or light green, nucleolus is indigo.
MucusSky-blue or pink.


  • 1. It is normal to produce a layer of oxide film on the surface of hematoxylin stain (Harris) and a little precipitation of aluminum sulfate crystals on the bottom. Before use, the oxide film should be removed and filtered regularly. Dilute lithium carbonate solution (a package of lithium carbonate powder is dissolved in 1000ml distilled water) is used for blueing.
  • 2. Hematoxylin stain (Harris) is not easy to stain when the temperature is low, and the staining time can be appropriately extended.
  • 3. The color separation is to use 1% hydrochloric acid alcohol solution to wash away the excessive hematoxylin absorbed by the cells, so that the nuclear and cytoplasmic contrast is distinct. The differentiation time should not be too long, otherwise the nucleus will be light stained.
  • 4. Orange G remained on the slide should be removed by washing in 95% ethanol. Otherwise, EA36 or EA50 Solution staining will be affected.
  • 5. Do not use the reagents beyond the stated expiration date. For storage, avoid exposure to extreme high or low temperature and sunlight.
  • 6. This reagent should be used by professionals and interpretation of the results. Read the instruction manual carefully before use, use it within the validity period, and do personal hygiene protection.
  • 7. Dispose of waste according to the requirements of the hospital or environmental protection department after use.
  • 8. The production date, production batch number and expiration date are shown in the outer packaging.

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