LPLC, TLC & Paper Chromatography

Introduction

Compared with high-pressure liquid chromatography, LPLC refers to liquid chromatography performed at a pressure close to the atmospheric pressure range. LPLC is used most frequently in the study of biomolecules such as proteins and monoclonal antibodies. Due to the fragile nature of biomolecules, LPLC is a useful option because it is a predominantly non-destructive, preparative technique in contrast to HPLC.

Thin layer chromatography (TLC) is to coat the suitable stationary phase on glass plate, plastic or aluminum substrate to form a uniform thin layer. After sampling and unfolding, the RF value is compared with the RF value of the chromatogram obtained by the control substance, which can be used for drug identification, impurity detection or content determination. TLC is an important experimental technique for rapid separation and qualitative analysis of small amounts of substances, and it is also used to track the reaction process.

Paper chromatography is a chromatographic method supported by filter paper, which is mainly used for the separation and detection of multi-functional or highly polar hydrophilic compounds such as alcohols, hydroxy acids, amino acids, sugars and flavonoids.

Alfa Chemistry's Chromatography Product Line

Alfa Chemistry's LPLC, TLC and Paper Chromatography product lines include resins, separation media, agarose, silica gel, dextran gel, etc. They are mainly used in the research of industrial preparation chromatography system and industrialization technology, such as desalination system, wastewater treatment, separation and purification of biological products, refining of sugar solution and so on. You can quickly find the materials you need by searching for keywords such as product name and CAS number.

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