Proteomic Products

• Protein Marker

Protein markers, also referred to as molecular weight markers, protein standards or protein ladders, are utilized to estimate the molecular weight of proteins resolved by gel electrophoresis. They are used to monitor protein migration during SDS-polyacrylamide gel electrophoresis, estimate the size of the target protein, and validate the Western transfer efficiency on PVDF, nylon, or nitrocellulose membran

• Western Substrate

ECL UltraScence Western Blotting Substrates refer to a series of luminol-based enhanced chemiluminescent substrates for the detection of antigen at different sensitivity levels. Western Substrate is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. It has a long chemiluminescent signal duration, making both digital and film-based imaging possible without any loss of the signal. Besides, optimal signal intensity and duration can be obtained by appropriate dilution of primary and secondary antibodies.

• Staining Reagent

Protein staining solution is used for SDS-PAGE gels to facilitate faster and more environmentally friendly gel visualization. And membrane stain is available for rapid and reversible protein staining on nitrocellulose or PVDF membranes to evaluate western blot transfer efficiency.

• OneStep Blocker

OneStep Blocker is a blocking solution for Western blot analysis that prevents different sources of background interference and serves as both a blocker and enhancer in Western analysis. Alfa Chemistry's OneStep Blocker buffer not only provides blocking and primary and secondary antibody hybridization in one step but also enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates.

• TMB ELISA Substrate

TMB ELISA Substrate, also known as 3,3',5,5'-tetramethylbenzidine, is a chromogenic substrate that detects horseradish peroxidase (HRP) activity. As a chromagen, TMB ELISA Substrate can detect horseradish peroxidase (HRP) activity when oxidized with hydrogen peroxide and yields a blue color with major absorbances at 370 and 652 nm.