RIBOAssure RNase Inhibitor

As a recombinant human placental protein expressed in Escherichia coli, the RIBOAssure RNase Inhibitor inhibits ribonuclease (RNase) activity exhibited by eukaryotic enzymes such as RNase A, RNase B, and RNase C by non-covalent binding. It is intended for use in applications where the presence of RNases is undesirable for obtaining optimal RNA quality and experiment results, for example, RNA isolation, cDNA synthesis, RT-PCR, in vitro transcription and translation, or RNase-free monoclonal antibody preparation. RIBOAssure RNase Inhibitor lacks activity towards RNase 1, RNase T1, RNase T2, S1 nuclease, and RNase H. It is highly recommended to be used with our high-performance RScript Reverse Transcriptase, qPCR MasterMixes, Taq DNA polymerase, and/or 2X PCR SuperMix for subsequent experimental steps and applications.

Kit Contents

Recommended Use

• RNA isolation and purification
• cDNA Synthesis, RT-PCR, RT-qPCR
• In Vitro Transcription

The optimal final concentration of the RIBOAssure RNase Inhibitor in a reaction would be recommended to in the range of 1-2 U/μl, depending on (1) the level of RNase contamination; (2) the incubation time; and (3) the compounds present in the reaction mixture. For the optimal activity, a final DTT concentration of 0.5–1 mM is essential.

The interference of RNase under the experimental conditions will reduce the cDNA quantity produced through RNA conversion, resulting in a decrease in the subsequent RT-qPCR's Ct value. After adding 4 units of RNase Inhibitor per reaction, the interference of RNase could be minimized.