Genomic DNA Isolation Kit (Paraffin-embedded tissue)

Catalog numberPDC12-0100

CategoriesColumn Based

Size100 Reactions

Sample4 rxns ($19.2)

$316.8 Inquire

Note: PureDireX / Purification Kits / Extraction Kit / Paraffin-embedded tissue

Description

The Genomic DNA Isolation Kit (Paraffin-embedded tissue) is designed specifically for genomic DNA isolation from animal tissue samples. This unique buffer system ensures total DNA with high yield and good quality from samples. The spin column system is designed to purify and concentrate DNA products which have been previously isolated using buffers. The entire procedure can be completed in 1 hour without phenol/chloroform extraction. Purified DNA is suitable for use in PCR or other enzymatic reactions.

Sample: 30 mg of fresh animal tissue.
Format: spin column.
Column capacity: up to 50 µg.
Operation time: within 60 minutes.

Kit Contents

Contents	PDC12-0100	PDC12-0100S (Sample)
Buffer TL	35 ml	1.5 ml
Buffer TP	45 ml	0.5 ml
Buffer W1	45 ml	2 ml
Buffer W2 (Add ethanol)	15 ml (60 ml)	300 µl × 2 (1.5 ml × 2)
Buffer BE	10 ml	1 ml
Column TC	100 pcs	4 pcs
Collection Tubes	100 pcs	4 pcs

Features

Delivering high-quality genomic DNA with the fast procedure.
Ready-to-use gnomic DNA for high performance in any downstream application.
Highly purified and high yield genomic DNA can be extracted from various samples.
Optimized lysis buffer for the efficient lysis.
Designed to rapidly purify high-quality DNA using spin column format.

Application

Gene cloning
PCR

Southern blotting
SNP genotyping

Protocol

Paraffin-embedded tissue

Genomic DNA Isolation Kit (Paraffin-embedded tissue)

Troubleshooting

Refer to the table below to troubleshoot problems that you may encounter when purifying the genomic DNA with the kit.

Problem	Cause	Solution
Low yield of DNA	Incomplete lysed sample	Use the appropriate method for the lysate preparation based on the amount of the starting materials.
		Increase the digestion time.
		Make sure that the tissue is completely immersed in the Buffer TL.
	Ethanol not added to Buffer W2	Add 60 ml of the ethanol (96–100%) to Buffer W2, and shake before use
	Incorrect elution conditions	Perform incubation at 75°C for 3 minutes with Buffer BE before centrifugation. To recover more DNA, perform a second elution step.
	Poor quality of starting material	Be sure to use fresh sample and process immediately after collection or freeze the sample at -80°C or in liquid nitrogen. The yield and quality of DNA isolated depends on the type and age of the starting material.
DNA degrade	Sample not fresh	Avoid repeated freeze / thaw cycles of the sample.
		Use a new sample for the DNA isolation. Perform the extraction of the fresh material when possible.
		Perform the extraction of the fresh material when possible.
	DNase contaminant	Use the fresh TAE or TBE electrophoresis buffer.
	DNase contaminant	Maintain a sterile work environment to avoid contamination from DNases.

Caution

During operation, always wear a lab coat, disposable gloves, and protective equipment.
Check buffers before use for salt precipitation. Redissolve any precipitate by warming to 37°C.
Add 60 ml of the ethanol (96–100%) to Buffer W2, and shake before use.
Buffers W1 contain irritants. Wear gloves when handling these buffers.
Research Use Only. Not intended for any animal or human therapeutic or diagnostic uses.

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