Genomic DNA Isolation Kit (Paraffin-embedded tissue)

Genomic DNA Isolation Kit (Paraffin-embedded tissue)
Genomic DNA Isolation Kit (Paraffin-embedded tissue)
Genomic DNA Isolation Kit (Paraffin-embedded tissue)

Catalog numberPDC12-0100

CategoriesColumn Based

Size100 Reactions

Sample4 rxns ($19.2)

$316.8 Inquire

Note: PureDireX / Purification Kits / Extraction Kit / Paraffin-embedded tissue


The Genomic DNA Isolation Kit (Paraffin-embedded tissue) is designed specifically for genomic DNA isolation from animal tissue samples. This unique buffer system ensures total DNA with high yield and good quality from samples. The spin column system is designed to purify and concentrate DNA products which have been previously isolated using buffers. The entire procedure can be completed in 1 hour without phenol/chloroform extraction. Purified DNA is suitable for use in PCR or other enzymatic reactions.

  • Sample: 30 mg of fresh animal tissue.
  • Format: spin column.
  • Column capacity: up to 50 µg.
  • Operation time: within 60 minutes.

Kit Contents

ContentsPDC12-0100PDC12-0100S (Sample)
Buffer TL35 ml1.5 ml
Buffer TP45 ml0.5 ml
Buffer W145 ml2 ml
Buffer W2 (Add ethanol)15 ml (60 ml)300 µl × 2 (1.5 ml × 2)
Buffer BE10 ml1 ml
Column TC100 pcs4 pcs
Collection Tubes100 pcs4 pcs
  • Delivering high-quality genomic DNA with the fast procedure.
  • Ready-to-use gnomic DNA for high performance in any downstream application.
  • Highly purified and high yield genomic DNA can be extracted from various samples.
  • Optimized lysis buffer for the efficient lysis.
  • Designed to rapidly purify high-quality DNA using spin column format.
  • Gene cloning
  • PCR
  • Southern blotting
  • SNP genotyping
  • Paraffin-embedded tissue

Genomic DNA Isolation Kit (Paraffin-embedded tissue)


Refer to the table below to troubleshoot problems that you may encounter when purifying the genomic DNA with the kit.

Low yield of DNAIncomplete lysed sampleUse the appropriate method for the lysate preparation based on the amount of the starting materials.
Increase the digestion time.
Make sure that the tissue is completely immersed in the Buffer TL.
Ethanol not added
to Buffer W2
Add 60 ml of the ethanol (96–100%) to Buffer W2, and shake before
Incorrect elution
Perform incubation at 75°C for 3 minutes with Buffer BE before
centrifugation. To recover more DNA, perform a second elution step.
Poor quality of
starting material
Be sure to use fresh sample and process immediately after
collection or freeze the sample at -80°C or in liquid nitrogen. The
yield and quality of DNA isolated depends on the type and age of
the starting material.
DNA degradeSample not freshAvoid repeated freeze / thaw cycles of the sample.
Use a new sample for the DNA isolation. Perform the extraction of the fresh material when possible.
Perform the extraction of the fresh material when possible.
DNase contaminantUse the fresh TAE or TBE electrophoresis buffer.
Maintain a sterile work environment to avoid contamination from DNases.
  • During operation, always wear a lab coat, disposable gloves, and protective equipment.
  • Check buffers before use for salt precipitation. Redissolve any precipitate by warming to 37°C.
  • Add 60 ml of the ethanol (96–100%) to Buffer W2, and shake before use.
  • Buffers W1 contain irritants. Wear gloves when handling these buffers.
  • Research Use Only. Not intended for any animal or human therapeutic or diagnostic uses.
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