Sample4 rxns ($19.2)
Sample4 rxns ($19.2)
Note: PureDireX / Purification Kits / Extraction Kit / Paraffin-embedded tissue
The Genomic DNA Isolation Kit (Paraffin-embedded tissue) is designed specifically for genomic DNA isolation from animal tissue samples. This unique buffer system ensures total DNA with high yield and good quality from samples. The spin column system is designed to purify and concentrate DNA products which have been previously isolated using buffers. The entire procedure can be completed in 1 hour without phenol/chloroform extraction. Purified DNA is suitable for use in PCR or other enzymatic reactions.
|Buffer TL||35 ml||1.5 ml|
|Buffer TP||45 ml||0.5 ml|
|Buffer W1||45 ml||2 ml|
|Buffer W2 (Add ethanol)||15 ml (60 ml)||300 µl × 2 (1.5 ml × 2)|
|Buffer BE||10 ml||1 ml|
|Column TC||100 pcs||4 pcs|
|Collection Tubes||100 pcs||4 pcs|
Refer to the table below to troubleshoot problems that you may encounter when purifying the genomic DNA with the kit.
|Low yield of DNA||Incomplete lysed sample||Use the appropriate method for the lysate preparation based on the amount of the starting materials.|
|Increase the digestion time.|
|Make sure that the tissue is completely immersed in the Buffer TL.|
|Ethanol not added|
to Buffer W2
|Add 60 ml of the ethanol (96–100%) to Buffer W2, and shake before|
|Perform incubation at 75°C for 3 minutes with Buffer BE before|
centrifugation. To recover more DNA, perform a second elution step.
|Poor quality of|
|Be sure to use fresh sample and process immediately after|
collection or freeze the sample at -80°C or in liquid nitrogen. The
yield and quality of DNA isolated depends on the type and age of
the starting material.
|DNA degrade||Sample not fresh||Avoid repeated freeze / thaw cycles of the sample.|
|Use a new sample for the DNA isolation. Perform the extraction of the fresh material when possible.|
|Perform the extraction of the fresh material when possible.|
|DNase contaminant||Use the fresh TAE or TBE electrophoresis buffer.|
|Maintain a sterile work environment to avoid contamination from DNases.|
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