Novel Juice

Novel Juice is a non-mutagenic fluorescent reagent producing an instant visualization of DNA bands upon illuminating agarose gels on the Blue Light (~470 nm) or UV Transilluminator (~302 nm). Novel Juice, as supplied in the 6X DNA Loading Buffer, is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visually tracking the DNA migration during the electrophoresis process and for detecting the double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and RNA as the most sensitive stain available on the market. The recommended DNA sample mass is at least 50 ng or more, thus not causing any obvious shift in the migration pattern.

It is the non-hazardous alternative to Ethidium Bromide for protecting the environment and meeting with the local biosafety regulations.

Reaction Setup

1. Vortex Novel Juice for 10 seconds prior to use.
2. Dilute 1 part of Novel Juice with 5 parts of DNA sample and mix. (Note: Novel Juice must be added to DNA markers in order to visualize the ladder bands simultaneously with the sample after electrophoresis).
3. Load sample and run according to standard procedures.
4. After the electrophoresis, remove gel and place it on UV or a visible -light transilluminator to immediately visualize bands.
5. Gels can be post-stained with Ethidium Bromide if desired.