Prime Juice Preloading Fluorescent Stain

Prime Juice is a non-mutagenic fluorescent reagent producing an instant visualization of DNA bands upon illuminating agarose gels on the Blue Light Transilluminator (~470 nm) or UV Transilluminator (~302 nm). Prime Juice, as supplied in the 6X DNA Loading Buffer, is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visually tracking the DNA migration during the electrophoresis process and for detecting the double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and RNA at as low as 0.14ng DNA, thus rendering it the most sensitive stain available on the market.

The recommended DNA sample mass is at least 50 ng or more, thus not causing any obvious shift in the migration pattern. Regardless of the loaded DNA or RNA sample amount for staining, it achieves cogently consistent migration pattern without the "smiley signature" commonly identified with other competing stains on the market. It is the ideal non-hazardous alternative to Ethidium Bromide for protecting the environment and meeting with the local biosafety regulations/ESG practices/UN SDG Goals.

Reaction Setup

1. Vortex Prime Juice for 10 seconds prior to use.
2. Dilute 1 part of Prime Juice with 5 parts of DNA sample and mix. (Note: Prime Juice must be added to DNA markers in order to visualize the ladder bands simultaneously with the sample after electrophoresis).
3. Load sample and run according to standard procedures.
4. After the electrophoresis, remove gel and place it on UV or a visible -light transilluminator to immediately visualize bands.
5. Gels can be post-stained with Ethidium Bromide if desired.