Bacterial Capsule Stain

Assay-kits

Product code: ACMBS-59
Specifications: 2x20mL (Box)
Intended use: Used for bacterial staining on whether contain the capsule structure
Store temperature: 5℃～30℃
Expiry date: 24 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationMethod limitationPrecautions

Principle

Capsule is a characteristic mucous substance developed external to but contagious with the cell wall during bacteria's life cycle. The capsule can protect bacteria from phagocytosis and subsequent digestion by themhost, thus increasing their infectivity. Capsules can only be stained with certain special stains. Identification of capsules is very important for bacteria grouping and differentiation.

Main components

Reagent composition	Main ingredient
1. Crystal violet solution	Crystal violet
2. Copper sulfate solution	Copper sulfate

Sample requirement

Pick colonies and make smear, ethanol-fixed after natural drying.

Test procedure

1. Add crystal violet solution, then heat on flame and keep staining solution steaming for about 1 minute.
2. Wash dyes on the smear with copper sulfate solution (avoid washing with water).
3. Dry with absorbent paper and observe under oil immersion lens.

Result interpretation

Bacteria and background	Purple
Capsule	Bright blue or unstained

Method limitation

Only use for morphological staining to observe.

Precautions

1. As water represents 90% of the capsule contents, do not fix samples by heating or capsules will shrink.
2. After staining, samples must be washed with copper sulfate solution instead of water.
3. This kit must be used by professionals and the interpretation of results.
4. Should carefully read the instruction manual before using. Can only be used before expiration date, and keep a good personal hygiene protection.
5. After use, disposal of waste should comply with the hospital or the environmental protection department requirements.
6. Tightly cap the reagent bottle immediately after use to avoid evaporation.
7. Production lot number and expiration date are on the package.

References

1. The PRC Ministry of Health Medical Administrative Department. National Clinical Laboratory Procedures (M) version 3. Nanjing: Southeast University Press, 2006.

2. Tong Mingqing: Clinical Laboratory Pathogenic Microbiology (M). Higher Education Press.

3. Ni Yuxing: Microbiology and microbiological testing experimental guidance (M). People's Health Publishing House.

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