Elastic Fiber Stain (Victoria Blue Method)

Assay-kits

Elastic Fiber Stain (Victoria Blue Method)

Product code: ACMBS-26
Specifications: 4x20mL, 4x100mL (Box)
Intended use: Used for tissue cytological staining to qualitatively detect elastic fibers in tissue sections
Store temperature: 5℃～30℃
Expiry date: 18 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationPrecautions

Principle

The principle of this method is not well-known. Hypothesis includes that hydrogen bond forms between certain parts of elastic fiber and phenolic group of resorcinol in Elastin Stain, consequently the elastic fiber is stained blue-black. VG stain was used for contrast stain.

Main components

Reagent composition	Main ingredient
1. Potassium permanganate solution	Potassium permanganate
2. Oxalic acid solution	Oxalic acid
3. Elastin stain	Victoria blue, resorcinol
4. Van Gieson stain	Acid fuchsin, picric acid

Sample requirement

The tissue section must be fully fixed.

Test procedure

1. The slices are routinely dewaxed to water and washed once with distilled water.
2. Oxidized in potassium permanganate solution for 5 minutes. Rinse in water.
3. Bleach in oxalic acid solution for 2～3 minutes. Rinse in water.
4. Rinse slightly in 95% ethanol and stain in Elastin stain for 8～24 hours (stamped, avoid volatile dye).
5. Differentiate in 95% ethanol or 1% hydrochloric acid alcohol (if necessary, under control of the microscope).
6. Rinse thoroughly in water for 3～5 minutes.
7. Counterstain in Van Gieson stain for 1 minute (without washing).
8. Differentiate quickly in 95% ethanol for several seconds.
9. Conventional dehydration and transparent, sealed with neutral resin.

Result interpretation

Elastic fiber	Blue-black
Collagen fiber	Red
Muscle fiber, erythrocyte	Yellow

Precautions

1. Differentiate tissue directly in 95% ethanol after Elastin stain, without rinsing in water, to avoid hard-to-differentiate.
2. As Elastin stain is easy volatile and takes a long time to complete the staining, please using dip stain in sealed container.
3. After staining in Van Gieson stain, do not rinse in water. Otherwise, the colors stained with Van Gieson stain will weaken or even discolor.
4. After the Elastin stain is unsealed, it should be placed in a cool, dark, airtight container, it can be reused until the stain solution fails.
5. Elastin stain can also be used 37℃ water bath (capped) staining 30～60 minutes to accelerate staining, but under the microscope control to prevent staining too deep, The stain solution after heating and used, the number of reusables will be reduced.
6. This reagent should be used by professionals and interpretation of the results. Read the instruction manual carefully before use, use it within the validity period, and do personal hygiene protection.
7. Dispose of waste according to the requirements of the hospital or environmental protection department after use.
8. The production date, production batch number and expiration date are shown in the outer packaging.

References

1. Chinese Medical Association. Clinical technical operating specifications-pathology volumes [M]. People's Military Medical Publishing House.

2. Ling Qibo. Practical pathology special staining and histochemistry technology [M]. Guangdong Higher Education Press.

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