Adrenal Chromaffin Cell Stain (Giemsa Method)

Assay-kits

PrincipleMain componentsSample requirementTest procedureResult interpretationPrecautions

After tissue fixed by containing chromium salt fixative, with Giemsa stain, the chromaffin cell granule was stained green-yellow.

Reagent composition	Main ingredient
1. Giemsa A solution	Giemsa dye
2. Giemsa B solution	Phosphate

Fresh tissue was immediately placed in Regaud's solution for 2 to 4 days, and the new solution was changed once a day, and then fixed with 3% potassium dichromate aqueous solution for 1 day, rinsed with running water for 24 hours, and dehydrated by conventional dehydration. The tissue section must be fully fixed.

1. The slices are routinely dewaxed to water and washed once with distilled water, about 30 minutes.
2. Placed into Giemsa diluent (A Solution: B solution = 1:20) dip stain 18～24 hours, washed with distilled water slightly and then dried with filter paper.
3. Differentiated few seconds with n-butanol (Purchase separately), then dried with filter paper.
4. Xylene transparent, sealed with neutral resin.

Chromaffin cells cytoplasmic granules	Green-yellow
Nucleus	Blue
Connective tissue	Light red

1. Regaud's fixative preparation: mix 3% potassium dichromate 80ml and 20ml concentrated formaldehyde solution before use, after mixed for 24 hours it will be gradually lapsed.
2. This reagent should be used by professionals and interpretation of the results. Read the instruction manual carefully before use, use it within the validity period, and do personal hygiene protection.
3. Dispose of waste according to the requirements of the hospital or environmental protection department after use.
4. The production date, production batch number and expiration date are shown in the outer packaging.

1. Chinese Medical Association. Clinical technical operating specifications - pathology volumes [M] People's Military Medical Publishing House.

2. Ling Qibo. Practical pathology special staining and histochemistry technology [M]. Guangdong Higher Education Press.

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