Alcian Blue Stain (pH 2.5)

Assay-kits

Alcian Blue Stain (pH 2.5)

Product code: ACMBS-21
Specifications: 2x20mL, 2x100mL (Box)
Intended use: Intended for containing sialic acid mucus and cryptococcus neoformans staining
Store temperature: 2℃～8℃
Expiry date: 18 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationPrecautions

Principle

Alcian blue is the most specific stain to detect acid mucous substances. This anion dye forms an electrovalent bond with acid group. Alcian blue can be used to separate and identify different mucous substances at various pH. At pH 1, carboxyl group (COOH) was not stained, sulfonic group (SO₃H) is stained, and at pH 2.5, carboxyl group is well stained, but poor stain of sulfate mucus.

Main components

Reagent composition	Main ingredient
1. Alcian blue solution (pH 2.5)	Alcian blue
2. Nuclear Fast Red solution	Nuclear Fast Red

Sample requirement

The tissue section must be fully fixed.

Test procedure

1. The slices are routinely dewaxed to water and washed once with distilled water.
2. Place in Alcian blue solution (pH 2.5) for 10～20 minutes and rinse briefly with water.
3. Counterstain in Nuclear Fast Red solution for 1～2 minutes and rinse briefly with water.
4. Conventional dehydration and transparent, sealed with neutral resin.

Result interpretation

Sialic acid and weakly sulfated mucilage	Blue
General mucus material	Blue
Nuclei and other tissues	Red
Capsule of Cryptococcus neoformans	Blue

Precautions

1. After removing the reagent from the refrigerator, please wait until it is fully restored to room temperature before use.
2. It is recommended to store the Nuclear Fast Red solution at room temperature for better coloring effect.
3. The Alcian blue solution is a strong stain with stable color. Overstaining is not a concern even after long staining.
4. Nuclear Fast Red solution staining longer the time, the Alcian blue coloring darker, or mucilage coagulation into a group, so you can also do not need to counterstained with Nuclear Fast Red solution.
5. This reagent should be used by professionals and interpretation of the results. Read the instruction manual carefully before use, use it within the validity period, and do personal hygiene protection.
6. Dispose of waste according to the requirements of the hospital or environmental protection department after use.
7. The production date, production batch number and expiration date are shown in the outer packaging.

References

1. Chinese Medical Association. Clinical technical operating specifications-pathology volumes [M]. People's Military Medical Publishing House.

2. Ling Qibo. Practical pathology special staining and histochemistry technology [M]. Guangdong Higher Education Press.

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