Helicobacter Pylori Stain (Methylene Blue Method)

Assay-kits

Helicobacter Pylori Stain (Methylene Blue Method)

Product code: ACMBS-22
Specifications: 20mL, 250mL (Box)
Intended use: Used for Helicobacter Pylori staining
Store temperature: 5℃～30℃
Expiry date: 18 Months

More Details

PrincipleMain componentsSample requirementTest procedureResult interpretationPrecautions

Principle

Helicobacter pylori is a gram-negative bacterium with short rod-shaped, both ends slightly bent. Helicobacter pylori mainly lives in the gastric mucosa epithelium and gastric gland mucus barrier, were closely related to chronic gastritis, peptic ulcer and gastric cancer. The common medical test of gastric Helicobacter pylori is traditional Silver nitrate method, but the reagent composition and operating procedures of method are more complex, but Baso Helicobacter pylori stain is a single reagent methylene blue method, can clearly show the Helicobacter Pylori.

Main components

Reagent composition	Main ingredient
Helicobacter pylori stain	Methylene blue

Sample requirement

The tissue section thickness 4～5μm and must be fully fixed.

Test procedure

1. The slices are routinely dewaxed to water and washed once with distilled water.
2. Place samples in Helicobacter pylori stain for 5～10 minutes.
3. Rinse with water.
4. Air dry, sealed with neutral resin, microscopic examination.

Result interpretation

Helicobacter pylori	Blue
Erythrocytes	Green
Background	Blue

Precautions

1. The slice after staining does not pass alcohol, because alcohol easily discolors the stained blue.
2. This reagent should be used by professionals and interpretation of the results. Read the instruction manual carefully before use, use it within the validity period, and do personal hygiene protection.
3. Dispose of waste according to the requirements of the hospital or environmental protection department after use.
4. The production date, production batch number and expiration date are shown in the outer packaging.

References

1. Chinese Medical Association. Clinical technical operating specifications-pathology volumes [M]. People's Military Medical Publishing House.

2. Ling Qibo. Practical pathology special staining and histochemistry technology [M]. Guangdong Higher Education Press.

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